Cell Signaling Technology

Product Pathways - Neuroscience

Musashi Antibody #2154

MSI   MSI1H   RNA-binding protein Musashi homolog 1   sc-98845  

No. Size Price
2154S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
2154 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 35 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Homology

Species predicted to react based on 100% sequence homology: Zebrafish,

Specificity / Sensitivity

Musashi Antibody detects endogenous levels of total Musashi 1 and 2 protein.

Musashi Antibody 兔单抗识别内源性的总Musashi 1和Musashi 2蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues of human Musashi. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体由合成的人类Musashi蛋白相应氨基酸序列对应肽段免疫动物而制成。该抗体使用蛋白A和多肽亲和层析纯化而得。

Western Blotting

Western Blotting

Western blot analysis of extracts from IEC-6 and LS174T cells, using Musashi Antibody.

Western blot分析IEC-6和LS174T细胞提取物,使用的抗体是Musashi Antibody 兔多抗。

IF-F

IF-F

Confocal immunofluorescence images of the subventricular zone of rat striatal sections labeled with Musashi Antibody (red) and Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (Alexa Fluor® 488 Conjugate) #4854 (green). Proliferating/mitotic cells are labeled with Phospho-Histone H3 (Ser10) (6G3) Mouse mAb #9706 (blue).

共聚焦免疫荧光分析大鼠脑室下纹状体区域,使用Musashi Antibody 兔多抗(红色)和Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (Alexa Fluor® 488 Conjugate) #4854(绿色)进行标记。增生/分裂细胞用 Phospho-Histone H3 (Ser10) (6G3) Mouse mAb 兔单抗#9706(蓝色)标记。

Background

Musashi-1 and Musashi-2 are RNA-binding proteins which play a role in asymmetric cell division of ectodermal precursor cells by regulating the translation of target mRNA. Both family members augment Notch signaling and repress the translation of m-Numb, a protein that positively modulates differentiation of neural stem cells into neurons. Thus, Musashi contributes to the maintenance of neural stem cells (1). While Musashi-1 is frequently used as a marker for proliferating neural precursor cells, it is also expressed in epithelial stem cells including intestinal and mammary gland stem cells (2-4).

Musashi-1和Musashi-2是RNA结合蛋白,通过调节靶mRNA的翻译在外胚层前体细胞的不对称细胞分裂中发挥作用。这两个成员都能增加Notch信号和抑制m-Numb的翻译;m-Numb是一种正向调节神经干细胞分化成神经元的蛋白质。因此,Musashi对神经干细胞的保存有贡献(1)。Musashi-1经常被用来作为神经前体细胞增殖的标记,它也在上皮干细胞,包括肠,乳腺干细胞中表达(2-4)。

  1. Okano, H. et al. (2005) Exp. Cell Res. 306, 349-56.
  2. Sakakibara, S. et al. (1996) Dev. Biol. 176, 230-42.
  3. Potten, C.S. et al. (2003) Differentiation 71, 28-41.
  4. Clarke, R.B. et al. (2005) Dev. Biol. 277, 443-56.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Alexa Fluor is a registered trademark of Life Technologies Corporation.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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