Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

WSTF Antibody #2152

No. Size Price
2152S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
2152 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 170, 185 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

WSTF Antibody detects endogenous levels of total WSTF protein. The antibody does not cross-react with other related transcription factors.

WSTF Antibody能够检测内源性WSTF总蛋白水平。该抗体不能与其它相关的转录因子发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human WSTF (WBSCR9). Antibodies are purified by protein A and peptide affinity chromatography.

通过人工合成人源WSTF (WBSCR9)蛋白相应的多肽片段去免疫动物从而制备出此多克隆抗体。通过蛋白A和多肽亲和层析纯化获得。

Western Blotting

Western Blotting

Western blot analysis of lysates from various cell types using WSTF Antibody.

使用WSTF Antibody,免疫印迹(Western blot)分析不同细胞中WSTF的蛋白水平。

Background

The human WSTF gene is located within the common Williams Syndrome (WS) deletion area at chromosome 7q11.23. Several WSTF gene products have been detected with little difference in length of polypeptides (1-3). Functional motifs identified by sequence-homology searches include a PHD-type zinc finger motif followed by a bromodomain. Both motifs are found in many transcription factors, suggesting that WSTF may function as a transcription factor. A Drosophila gene (acf1) was cloned, which encodes two forms of Acf1 proteins with molecular weight 170 kDa and 185 kDa, respectively (4). It was demonstrated that Acf1 is structurally related to the human WSTF gene. Acf1 forms a complex with another protein, ISWI, and functions in the ATP-dependent catalysis of chromatin assembly (4).

人源WSTF基因定位在染色体7q11.23的普通威廉姆斯综合症 (Williams Syndrome,WS)截断区域。数个WSTF基因表达产物的多肽片段中很小的区别已经被检测(1-3)。序列同源性搜索鉴定功能性结构域,包括一个PHD-type锌指结构和随后的bromodomain。两个结构域都被发现在许多转录因子中,这被认为WSTF可能作为一个转录因子。一个果蝇基因(acf1) 被克隆,这编码Acf1蛋白的两个亚型,其分子量分别为170 kDa和185 kDa (4)。研究证明Acf1与人源WSTF基因在结构上相关。Acf1形成以各含另外蛋白ISWI的复合物,并且其功能是在染色质装配的ATP依赖的催化作用中扮演着角色(4)。

  1. Peoples, R.J. et al. (1998) Cytogenet. Cell Genet. 82, 238-246.
  2. Lu, X. et al. (1998) Genomics 54, 241-249.
  3. Jones, M.H. et al. (2000) Genomics 63, 40-45.
  4. Ito, T. et al. (2000) Genes Dev. 13, 1529-1539.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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