Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

α/β-Tubulin Antibody #2148

a-tubulin   Alpha-tubulin ubiquitous   anti-tubulin   b-tubulin   sc-5546   TBAK   TBB2   TUBB   TUBB2   TUBB5   tublin   Tubulin alpha-ubiquitous chain   Tubulin beta-2 chain   Tubulin K-alpha-1   tubuline   βtubulin  

No. Size Price
2148S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
2148 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey,Zebrafish,Bovine, Endogenous 55 Rabbit
IHC-P 1:100
F 1:50
IF-IC 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

The α/β-Tubulin Antibody detects endogenous levels of both α- and β-tubulin total protein, and does cross-react with both recombinant α- and β-tubulin.

α/β-Tubulin Antibody检测内源性α-和β-tubulin总蛋白,而且不与重组的α-和β-tubulin蛋白发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human α- and β-tubulin. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3, C6 and COS-7 cells, using α/β-Tubulin Antibody.

使用α/β-Tubulin Antibody,免疫印迹(Western Blot)分析HeLa、NIH/3T3、C6和COS-7细胞中α/β-Tubulin蛋白水平。

Western Blotting

Western Blotting

Western blot analysis of recombinant alpha-tubulin and beta-tubulin GST-fusion proteins, and extracts from NIH/3T3 and C6 cells, using α/β-Tubulin Antibody (left) and GST Antibody #2622 (right).

使用α/β-Tubulin Antibody (左图)和GST Antibody #2622 (右图),免疫印迹(Western Blot)分析NIH/3T3和C6细胞中重组的alpha-tubulin and beta-tubulin GST-fusion蛋白水平。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of C6 cells using α/β-Tubulin Antibody (blue) compared to a nonspecific negative control antibody (red).

与非特异阴性control antibody (红色)比较,使用α/β-Tubulin Antibody (蓝色)标记进行流式细胞仪分析C6细胞。



Confocal microscopic images of NIH/3T3 cells showing cytoskeletal stain with α/β-Tubulin Antibody (A) compared to an isotype control (B).

与an isotype control (B)比较,使用α/β-Tubulin Antibody (A),共聚焦免疫荧光观察NIH/3T3细胞,结果显示细胞骨架着色。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, showing cytoplasmic localization, using α/β-Tubulin Antibody.

使用α/β-Tubulin Antibody,免疫组化分析人源结肠癌组织石蜡切片,结果显示细胞质定位。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using α/β-Tubulin Antibody.

使用α/β-Tubulin Antibody,免疫组化分析人源乳腺癌组织石蜡切片。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human MALToma using α/β-Tubulin Antibody.

使用α/β-Tubulin Antibody,免疫组化分析人源淋巴瘤组织石蜡切片。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using β-Tubulin (9F3) Rabbit mAb #2128 in the presence of control peptide (left) or β-Tubulin Blocking Peptide (right).

使用β-Tubulin (9F3) Rabbit mAb #2128,并且提前与control peptide (左图)或β-Tubulin Blocking Peptide (右图)孵育,免疫组化分析人源乳腺癌组织石蜡切片。



Confocal immunofluorescent analysis of NIH-3T3 cells, using α/β-Tubulin Antibody (green) and Phospho-Histone H3 (Ser10) (6G3) Mouse mAb #9706 (red) showing different stages of the cell cycle.

使用α/β-Tubulin Antibody (绿色)和Phospho-Histone H3 (Ser10) (6G3) Mouse mAb #9706 (红色),共聚焦免疫荧光观察NIH/3T3细胞,结果显示细胞周期的不同阶段。


The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).


  1. Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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