Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

β-Tubulin Antibody #2146

b-tubulin   sc-9104   sc-9935   TBB2   TUBB   TUBB2   TUBB5   βtubulin  

No. Size Price
2146S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
2146 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey,Zebrafish,Bovine, Endogenous 55 Rabbit
IHC-P 1:100
F 1:100
IF-IC 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Homology

Species predicted to react based on 100% sequence homology: Xenopus,

Specificity / Sensitivity

The β-Tubulin Antibody detects endogenous levels of total β-tubulin protein, and does not cross-react with recombinant α-tubulin.

β-Tubulin Antibody检测内源性β-tubulin总蛋白,而且不与重组的α-tubulin蛋白发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human β-tubulin. Antibodies are purified by protein A and peptide affinity chromatography.

通过人工合成人源β-tubulin蛋白相应的多肽片段去免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。

Western Blotting

Western Blotting

Western blot analysis of extracts from CAD and C6 cells using β-Tubulin Antibody.

使用β-Tubulin Antibody,免疫印迹(Western Blot)分析CAD和C6细胞中β-Tubulin蛋白水平。

Western Blotting

Western Blotting

Western blot analysis of recombinant alpha-tubulin and beta-tubulin GST-fusion proteins and extracts from CAD cells using α-Tubulin Antibody #2144(left), β-Tubulin Antibody (middle) and GST Antibody #2622 (right).

使用α-Tubulin Antibody #2144(左图)、β-Tubulin Antibody (中间图)和GST Antibody #2622 (右图),免疫印迹(Western Blot)分析CAD细胞中alpha-tubulin和beta-tubulin GST-fusion蛋白水平。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of C6 cells, using β-Tubulin Antibody (blue) compared to a nonspecific negative control antibody (red).

与非特异阴性control antibody (红色)比较,使用β-Tubulin Antibody (蓝色)标记进行流式细胞仪分析C6细胞。

IF-IC

IF-IC

Confocal microscopic images of NIH/3T3 cells showing cytoskeletal stain with β-Tubulin Antibody (A) compared to an isotype control (B).

与an isotype control (B)比较,使用β-Tubulin Antibody (A),共聚焦免疫荧光观察NIH/3T3细胞。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic localization, using β-Tubulin Antibody.

使用β-Tubulin Antibody,免疫组化分析人源乳腺癌组织石蜡切片。结果显示细胞质定位。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using β-Tubulin Antibody.

使用β-Tubulin Antibody,免疫组化分析人源结肠癌组织石蜡切片。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human MALToma, using β-Tubulin Antibody.

使用β-Tubulin Antibody,免疫组化分析人源淋巴瘤组织石蜡切片。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using β-Tubulin (9F3) Rabbit mAb #2128 in the presence of control peptide (left) or β-Tubulin Blocking Peptide (right).

使用β-Tubulin (9F3) Rabbit mAb #2128,并且提前与control peptide (左图)或β-Tubulin Blocking Peptide (右图)孵育,免疫组化分析人源乳腺癌组织石蜡切片。

Background

The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).

细胞骨架由三种类型的细胞质基质纤维组成:微管、微丝(肌动蛋白纤维)和中间纤维。球形微管蛋白亚基包含微管建筑块,由α/β-tubulin异源二聚体形成所有真核细胞的tubulin亚单位。γ-tubulin被需要在tubulin亚单位的成核聚合作用中去形成微管聚合物。许多细胞运动是通过微管动力介导的,包括纤毛和鞭毛的跳动、膜泡的核质转运、在减数分裂/有丝分裂期间染色体排布和神经轴突的迁移。这些运动由于竞争性微管聚合作用和解聚作用或通过微管动力蛋白的运动(1)。

  1. Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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