Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

α-Tubulin Antibody #2144

a-tubulin   Alpha-tubulin ubiquitous   sc-5546   TBAK   Tubulin alpha-ubiquitous chain   Tubulin K-alpha-1  

No. Size Price
2144S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
2144 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey,D. melanogaster,Bovine, Endogenous 52 Rabbit
IHC-P 1:50
F 1:25
IF-IC 1:25

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),


Species predicted to react based on 100% sequence homology: Xenopus,

Specificity / Sensitivity

The α-Tubulin Antibody detects endogenous levels of total α-tubulin protein, and does not cross-react with recombinant β-tubulin.

α-Tubulin Antibody检测内源性α-tubulin总蛋白,而且不与重组的β-tubulin蛋白发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human α-tubulin. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from CAD and C6 cells, using α-Tubulin Antibody.

使用α-Tubulin Antibody,免疫印迹(Western Blot)分析CAD和C6细胞中α-Tubulin蛋白水平。

Western Blotting

Western Blotting

Western blot analysis of recombinant α-tubulin and β-tubulin GST-fusion proteins, and extracts from CAD cells, using α-Tubulin Antibody (left), β-Tubulin Antibody #2146 (middle) and GST Antibody #2622 (right).

使用α-Tubulin Antibody (左图)、β-Tubulin Antibody #2146 (中间图)和GST Antibody #2622 (右图),免疫印迹(Western Blot)分析CAD细胞中重组的α-Tubulin和β-tubulin GST-fusion蛋白水平。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of C6 cells, using α-Tubulin Antibody (blue) compared to a nonspecific negative control antibody (red).

与非特异阴性control antibody (红色)比较,使用α-Tubulin Antibody (蓝色)标记进行流式细胞仪分析C6细胞。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic localization using α-Tubulin Antibody.

使用α-Tubulin Antibody,免疫组化分析人源乳腺癌组织石蜡切片,结果显示定位细胞质。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using α-Tubulin Antibody.

使用α-Tubulin Antibody,免疫组化分析人源结肠癌组织石蜡切片。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human Non-Hodgkin's lymphoma, using α-Tubulin Antibody.

使用α-Tubulin Antibody,免疫组化分析人源Non-Hodgkin's lymphoma组织石蜡切片。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using α-Tubulin Antibody in the presence of control peptide (left) or antigen-specific peptide (right).

使用α-Tubulin Antibody,并且提前与control peptide (左图)或antigen-specific peptide (右图)孵育,免疫组化分析人源乳腺癌组织石蜡切片。



Confocal immunofluorescent analysis of NIH/3T3 cells, using α-Tubulin Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

使用α-Tubulin Antibody (绿色)标记,共聚焦免疫荧光观察α-Tubulin蛋白在NIH/3T3细胞中定位。Alexa Fluor® 555 phalloidin标记微丝蛋白(红色)。蓝色伪彩=DRAQ5®#4084(DNA荧光染料)。


The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).


  1. Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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