Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

LSD1 Antibody #2139

Amine oxidase flavin containing domain protein 2   AOF2   KIAA0601   Lysine-specific histone demethylase 1   np90   p110b   sc-271720  

No. Size Price
2139S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
2139 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 110 Rabbit
IP 1:50
IHC-P 1:200
F 1:400
IF-IC 1:400
ChIP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry), ChIP=Chromatin IP,

Specificity / Sensitivity

LSD1 Antibody detects endogenous levels of total LSD1 protein.

LSD1 Antibody能够检测内源性LSD1总蛋白水平。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino-terminus of human LSD1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

通过合成的与人源LSD1蛋白氨基端相应多肽片段去免疫动物从而制备此多克隆抗体。通过蛋白蛋白A和多肽亲和层析纯化获得。

Western Blotting

Western Blotting

Western blot analysis of cell lysates from various cell lines, using LSD1 Antibody.

使用LSD1 Antibody,免疫印迹(Western blot)分析不同细胞中LSD1的蛋白水平。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of untreated HeLa cells, using LSD1 Antibody (blue) compared to a nonspecific negative control antibody (red).

与非特异阴性control antibody (红色)比较,使用LSD1 Antibody (蓝色)标记,流式细胞仪分析未处理的HeLa细胞。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma showing nuclear localization, using LSD1 Antibody.

使用LSD1 Antibody,免疫组化分析人源肺癌组织石蜡切片,结果显示为细胞核定位。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical staining of paraffin-embedded human breast carcinoma, using LSD1 Antibody preincubated with control peptide (left) or antigen-specific peptide (right).

使用LSD1 Antibody,左图:control peptide, 右图: antigen-specific peptide,免疫组化分析人源乳腺癌组织石蜡切片。

IF-IC

IF-IC

Immunofluorescent analysis of HeLa cells, using LSD1 Antibody (right). Nuclei are stained with DAPI (left).

使用LSD1 Antibody (右图),免疫荧光分析HeLa细胞。DAPI染细胞核(左图)。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 U-937 cells and either 10 μl of LSD1 Antibody or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR SimpleChIP® Human CD11b Promoter Primers #14271, human SPI-1 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Background

Lysine-specific demethylase 1 (LSD1; also known as AOF2 and BHC110) is a nuclear amine oxidase homolog that acts as a histone demethylase and transcription cofactor (1). Gene activation and repression is specifically regulated by the methylation state of distinct histone protein lysine residues. For example, methylation of histone H3 at Lys4 facilitates transcriptional activation by coordinating the recruitment of BPTF, a component of the NURF chromatin remodeling complex, and WDR5, a component of multiple histone methyltransferase complexes (2,3). In contrast, methylation of histone H3 at Lys9 facilitates transcriptional repression by recruiting HP1 (4,5). LSD1 is a component of the CoREST transcriptional co-repressor complex that also contains CoREST, CtBP, HDAC1 and HDAC2. As part of this complex, LSD1 demethylates mono-methyl and di-methyl histone H3 at Lys4 through a FAD-dependent oxidation reaction to facilitate neuronal-specific gene repression in non-neuronal cells (1,6,7). In contrast, LSD1 associates with androgen receptor in human prostate cells to demethylate mono-methyl and di-methyl histone H3 at Lys9 and facilitate androgen receptor-dependent transcriptional activation (8). Therefore, depending on gene context LSD1 can function as either a transcriptional co-repressor or co-activator. LSD1 activity is inhibited by the amine oxidase inhibitors pargyline, deprenyl, clorgyline and tranylcypromine (8).

Lysine-specific demethylase 1 (LSD1; 也称为AOF2和BHC110)是一个核胺氧化酶同系物,它能作为一个组蛋白去甲基化和转录辅因子(1)。明显的组蛋白赖氨酸残基的甲基化水平能够特异性调节基因激活和抑制。例如,histone H3蛋白Lys4位点的甲基化通过协调BPTF和WDR5的招募有助于转录激活,而BPTF是NURF染色质重塑复合物的成分,并且WDR5是多种组蛋白甲基转移酶复合物成分(2,3)。与此相反,histone H3蛋白Lys9位点甲基化通过招募HP1有助于转录抑制(4,5)。LSD1是CoREST转录辅助抑制因子复合物的成分,该复合物也包含CoREST、CtBP、HDAC1和HDAC2。作为这个复合物的一部分,在非神经元细胞中LSD1使单甲基化和双甲基化的histone H3蛋白Lys4位点去甲基化,这通过一个FAD依赖的氧化反应去有助于神经特异性基因的抑制(1,6,7)。相反,在人源前列腺细胞中LSD1与雄激素受体有关联,从而使单甲基化和双甲基化histone H3蛋白Lys9位点去甲基化,以及有助于雄激素受体依赖的转录激活(8)。依据基因背景,LSD1的功能既可以是转录的辅助抑制因子,又可以使辅助激活因子。LSD1活性通过胺氧化酶抑制剂pargyline、deprenyl、clorgyline和tranylcypromine被抑制(8)。

  1. Shi, Y. et al. (2004) Cell 119, 941-953.
  2. Wysocka, J. et al. (2006) Nature 442, 86-90.
  3. Wysocka, J. et al. (2005) Cell 121, 859-872.
  4. Jacobs, S.A. and Khorasanizadeh, S. (2002) Science 295, 2080-2083.
  5. Nielsen, P.R. et al. (2002) Nature 416, 103-107.
  6. Shi, Y.J. et al. (2005) Mol. Cell 19, 857-864.
  7. Lee, M.G. et al. (2005) Nature 437, 432-435.
  8. Metzger, E. et al. (2005) Nature 437, 436-439.

Application References

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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