Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

β-Tubulin (9F3) Rabbit mAb #2128

sc-5274   sc-55529   sc-9104   TBB2   TUBB   TUBB2   TUBB5   Tubulin beta-2 chain   βtubulin  

No. Size Price
2128L 300 µl ( 30 western blots ) ¥7,504.00 现货查询 购买询价
2128S 100 µl ( 10 western blots ) ¥3,000.00 现货查询 购买询价
2128T 20 µl ( 2 western blots ) ¥1,200.00 现货查询 购买询价
2128 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey,Zebrafish,Bovine, Endogenous 55 Rabbit IgG
IHC-P 1:50
F 1:100
IF-IC 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Homology

Species predicted to react based on 100% sequence homology: Chicken,

Specificity / Sensitivity

β-Tubulin (9F3) Rabbit mAb detects endogenous levels of total β-tubulin protein, and does not cross-react with recombinant α-tubulin.

β-Tubulin (9F3) Rabbit mAb兔单抗检测内源性β-tubulin总蛋白,而且不与重组的α-tubulin蛋白发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of human β-tubulin.

通过人工合成人源β-tubulin蛋白的氨基端相应的多肽片段去免疫动物从而制备出单克隆抗体。

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells, using β-Tubulin (9F3) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

使用β-Tubulin (9F3) Rabbit mAb(绿色)标记,共聚焦免疫荧光观察β-Tubulin蛋白在HeLa细胞中定位。蓝色伪彩=DRAQ5®#4084(DNA荧光染料)。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of NIH/3T3 cells using β-Tubulin (9F3) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).

与非特异阴性control antibody (红色)比较,使用β-Tubulin (9F3) Rabbit mAb (蓝色)标记进行流式细胞仪分析NIH/3T3细胞。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using β-Tubulin (9F3) Rabbit mAb.

使用β-Tubulin (9F3) Rabbit mAb兔单抗,免疫组化分析人源肺癌组织石蜡切片。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using β-Tubulin (9F3) Rabbit mAb preincubated with control peptide (left) or β-Tubulin Blocking Peptide #1032 (right).

使用β-Tubulin (9F3) Rabbit mAb,并且提前与control peptide (左图)或β-Tubulin Blocking Peptide #1032 (右图)孵育,免疫组化分析人源乳腺癌石蜡切片。

Western Blotting

Western Blotting

Western blot analysis of extracts from COS-7, NIH/3T3 and PC12 cells, using β-Tubulin (9F3) Rabbit mAb.

使用β-Tubulin (9F3) Rabbit mAb,免疫印迹(Western Blot)分析COS-7、NIH/3T3和PC12细胞中β-Tubulin蛋白水平。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human melanoma, using β-Tubulin (9F3) Rabbit mAb.

使用β-Tubulin (9F3) Rabbit mAb,免疫组化分析黑素瘤组织石蜡切片。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human glioblastoma, using β-Tubulin (9F3) Rabbit mAb.

使用β-Tubulin (9F3) Rabbit mAb,免疫组化分析人源恶性胶质瘤组织石蜡切片。

Background

The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).

细胞骨架由三种类型的细胞质基质纤维组成:微管、微丝(肌动蛋白纤维)和中间纤维。球形微管蛋白亚基包含微管建筑块,由α/β-tubulin异源二聚体形成所有真核细胞的tubulin亚单位。γ-tubulin被需要在tubulin亚单位的成核聚合作用中去形成微管聚合物。许多细胞运动是通过微管动力介导的,包括纤毛和鞭毛的跳动、膜泡的核质转运、在减数分裂/有丝分裂期间染色体排布和神经轴突的迁移。这些运动由于竞争性微管聚合作用和解聚作用或通过微管动力蛋白的运动(1)。

  1. Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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