Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

α-Tubulin (11H10) Rabbit mAb #2125

a-tubulin   Alpha-tubulin ubiquitous   K-ALPHA-1 alpha tubulin {K-ALPHA-1}   sc-398103   sc-5546   sc-8035   TBAK   Tubulin alpha-ubiquitous chain   Tubulin K-alpha-1  

No. Size Price
2125S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
2125 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey,D. melanogaster,Zebrafish,Bovine,Pig, Endogenous 52 Rabbit IgG
IHC-P 1:50
F 1:100
IF-IC 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Homology

Species predicted to react based on 100% sequence homology: Dog,

Specificity / Sensitivity

α-Tubulin (11H10) Rabbit mAb detects endogenous levels of total α-tubulin protein, and does not cross-react with recombinant β-tubulin.

α-Tubulin (11H10) Rabbit mAb兔单抗检测内源性α-tubulin总蛋白,但是不能与重组的β-tubulin蛋白发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of human α-tubulin protein.

通过人工合成人源α-tubulin蛋白的氨基末端相应的多肽片段去免疫动物从而制备出单克隆抗体。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells, using α-Tubulin (11H10) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).

与nonspecific negative control antibody (红色)比较,使用α-Tubulin (11H10) Rabbit mAb兔单抗 (蓝色)标记,流式细胞仪分HeLa细胞。

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells, using α-Tubulin (11H10) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

使用α-Tubulin (11H10) Rabbit mAb 兔单抗(绿色)标记,共聚焦免疫荧光分析HeLa细胞。Alexa Fluor® 555 phalloidin标记微丝蛋白(红色)。蓝色伪彩= DRAQ5® #4084 (DNA荧光染料)。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human melanoma, using α-Tubulin (11H10) Rabbit mAb.

使用α-Tubulin (11H10) Rabbit mAb兔单抗,免疫组化分析人源黑色素瘤组织石蜡切片。

Western Blotting

Western Blotting

Western blot analysis of extracts from C6, COS-7, NIH/3T3 and HeLa cells, using α-Tubulin (11H10) Rabbit mAb.

使用α-Tubulin (11H10) Rabbit mAb兔单抗,免疫印迹(Western Blot)分析C6、COS-7、NIH/3T3 和HeLa细胞中α-Tubulin蛋白水平。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human glioblastoma, using α-Tubulin (11H10) Rabbit mAb. 使用α-Tubulin (11H10) Rabbit mAb,免疫组化分析人源恶性胶质细胞瘤组织石蜡切片。

Background

The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).

细胞骨架由三种类型的细胞质基质纤维组成:微管、微丝(肌动蛋白纤维)和中间纤维。球形微管蛋白亚基包含微管建筑块,由α/β-tubulin异源二聚体形成所有真核细胞的tubulin亚单位。γ-tubulin被需要在tubulin亚单位的成核聚合作用中去形成微管聚合物。许多细胞运动是通过微管动力介导的,包括纤毛和鞭毛的跳动、膜泡的核质转运、在减数分裂/有丝分裂期间染色体排布和神经轴突的迁移。这些运动由于竞争性微管聚合作用和解聚作用或通过微管动力蛋白的运动(1)。

  1. Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47.

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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