Cell Signaling Technology

Product Pathways - Apoptosis

Cleaved α-Fodrin (Asp1185) Antibody #2121


No. Size Price
2121S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2121 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 150 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Cleaved alpha-Fodrin (Asp1185) Antibody detects endogenous levels of the large fragment of alpha-fodrin resulting from cleavage at aspartic acid 1185. The antibody does not recognize full length alpha-fodrin.

Cleaved alpha-Fodrin (Asp1185) Antibody 能够检测内源性水平Asp1185处剪切的α-胞衬蛋白大片段。该抗体不能够识别全长α-胞衬蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues surrounding Asp1185 in human alpha-fodrin. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体是采用合成的人源α-胞衬蛋白 Asp1185 氨基末端残基相对应的肽段免疫动物而制备的。抗体由protein A和肽亲和层析技术纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated or staurosporine-treated, using Cleaved alpha-Fodrin (Asp1185) Antibody (left) or alpha-Fodrin Antibody #2122 (right).

Western blot方法检测HeLa细胞提取物,未经处理或staurosporine处理,使用抗体为Cleaved alpha-Fodrin (Asp1185) Antibody (左图)或alpha-Fodrin Antibody #2122 (右图)。


Fodrin (also named nonerythroid spectrin) is a universally expressed membrane-associated cytoskeletal protein consisting of alpha- and beta-subunits (1). This protein is important for maintaining normal membrane structure and supporting cell surface protein function (1). Alpha-fodrin is one of the primary targets cleaved by caspases during apoptosis. The full length 240 kDa protein can be cleaved at several sites within its sequence by activated caspases to yield amino-terminal 150 kDa, carboxy-terminal 120 kDa and 35 kDa major products (2-5). Cleavage of alpha-fodrin leads to membrane malfunction and cell shrinkage.

Fodrin 胞衬蛋白(来自非红细胞,但与血影蛋白类似)是普遍表达的膜相关的细胞骨架蛋白,由α-和β-亚基组成(1)。这种蛋白质对于维持正常的膜结构和支持细胞表面蛋白的功能是很重要的(1)。α-胞衬蛋白在细胞凋亡过程中是半胱天冬酶裂解的主要靶点之一。这个全长240 kDa的蛋白可以在几个位点内被激活的半胱天冬酶裂解,产生150 kDa的氨基末端,120 kDa的羧基末端和35 kDa的主要产物(2-5)。α-胞衬蛋白的裂解导致膜故障和细胞收缩。

  1. Bennett, V. and Gilligan, D.M. (1993) Annu. Rev. Cell Biol. 9, 27-66.
  2. Vanags, D. M. et al. (1996) J. Biol. Chem. 271, 31075-31085.
  3. Cryns, V. L. et al. (1996) J. Biol. Chem. 271, 31277-31282.
  4. Wang, K. K. et al. (1998) J. Biol. Chem. 273, 22490-22497.
  5. Janicke, R. U. et al. (1998) J. Biol. Chem. 273, 15540-15545.

Application References

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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