Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) #2116


Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
IF-IC 1:200 Human,Mouse,Rat,Monkey,Zebrafish,Bovine, Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: IF-IC=Immunofluorescence (Immunocytochemistry),


Species predicted to react based on 100% sequence homology: Chicken,

Specificity / Sensitivity

β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) detects endogenous levels of total β-tubulin protein.

β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate)兔单抗检测内源性β-tubulin总蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to the amino terminus of human β-tubulin. The antibody was conjugated to Alexa Fluor® 555 under optimal conditions with an F/P ratio of 2-6.

通过人工合成人源β-tubulin蛋白的氨基端相应的磷酸化片段去免疫动物从而制备出单克隆抗体。该抗体在F/P比值2-6的最佳条件下连接Alexa Fluor® 555。


Cell Signaling Technology antibody is conjugated to Alexa Fluor® 555 fluorescent dye and tested in-house for direct immunofluorescent analysis of human and monkey cells. The unconjugated antibody #2128 reacts with human, mouse, rat, monkey, bovine, zebrafish and fly β-tubulin protein. CST expects that β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) will also recognize β-tubulin in these species.

Cell Signaling Technology抗体连接着Alexa Fluor® 555 fluorescent dye,并且在人源和猴细胞中直接用免疫荧光分析方法进行内部检测。非连接抗体#2128可与人源、小鼠、大鼠、猴、牛、斑马鱼和苍蝇的β-tubulin蛋白发生反应。CST期望β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate)在这些物种中识别β-tubulin蛋白。



Confocal immunofluorescent analysis of HeLa cells, serum-starved (left) or 20% serum-treated (right), using β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) (red) and Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (Alexa Fluor® 488 Conjugate) #4854 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

使用β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) (红色)和Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (Alexa Fluor® 488 Conjugate) #4854 (绿色)标记,共聚焦免疫荧光观察β-Tubulin和Phospho-S6 Ribosomal Protein蛋白在HeLa细胞中定位,细胞分为serum-starved (左图) or 20% serum-treated (右图)。蓝色伪彩=DRAQ5®#4084(DNA荧光染料)。


The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).


  1. Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

The Alexa Fluor dye antibody conjugates in this product are sold under license from Life Technologies Corporation for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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