Cell Signaling Technology

Product Pathways - DNA Damage

Cycloheximide #2112

apoptosis   bortezomib   CHX   cleaved parp   protein synthesis inhibitor   TNF   Tumor Necrosis Factor   ubiquitin  

Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Molecular Characterization






Chemical structure of cycloheximide.放线菌酮的化学结构。

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat cells, untreated (-) , or treated with Cycloheximide (50 μg/ml, 24 hr; +), Bortezomib #2204 (10 nM, 24 hr; +), or both, using Ubiquitin Antibody #3933 (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).Western blot分析Jurkat细胞提取物,未处理(-)或用放线菌酮(50 μg/ml, 24小时; +),Bortezomib #2204 (10 nM, 24小时; +)处理,或同时用两者处理,使用的抗体是:Ubiquitin Antibody #3933(上图)、β-Actin (D6A8) Rabbit mAb #8457(下图)。

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat cells, untreated (-) or treated with increasing concentrations of Cycloheximide (24 hr), using PARP Antibody #9542 (upper), Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb #5625 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower).Western blot分析Jurkat细胞提取物,未处理(-)或用浓度不断增加的放线菌酮(24小时,+)处理,使用的抗体是:PARP Antibody #9542(上图)、Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb #5625(中间)、β-Actin (D6A8) Rabbit mAb #8457(下图)。

Directions for Use

Cycloheximide is supplied as a lyophilized powder. For a 10 mg/ml stock, carefully weigh out and reconstitute 50 mg in 5 ml DMSO or EtOH. Working concentrations and length of treatments vary depending on the desired effect, but it is typically used at 5-50 µg/ml for 4-24 hours. Soluble in DMSO, EtOH, or MeOH.

提供的放线菌酮为冻干粉。对于10 mg/ml浓度贮存液,在50 mg冻干粉中加入5 ml DMSO或乙醇。工作浓度和处理时间根据所需的预期效果来确定,一般是5-50 µg/ml,处理4-24小时。能溶于DMSO、乙醇和甲醇。


Cycloheximide is a protein synthesis inhibitor in eukaryotes. Although its precise mechanism of action has yet to be fully elucidated, it has been shown to inhibit translation elongation through binding to the E-site of the 60S ribosomal unit and interfering with deacetylated tRNA (1-3). Although not all cell types are equally sensitive to the apoptosis-inducing effects of cycloheximide, it has been shown to induce cell death in T cells through a FADD-dependent mechanism (4). In addition, cycloheximide and Tumor Necrosis Factor possess a synergistic cytotoxicity (5,6), and consequently they are routinely used together to induce cell death. Investigators have demonstrated that cycloheximide blocks bortezomib-stimulated protein ubiquitination (7).


Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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