Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-Src (Tyr527) Antibody #2105

No. Size Price
2105S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2105T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
2105 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 60 Rabbit
IHC-P 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin),

Homology

Species predicted to react based on 100% sequence homology: Chicken,

Specificity / Sensitivity

Phospho-Src (Tyr527) Antibody detects endogenous levels of Src only when phosphorylated at Tyr527. The antibody may cross-react with other Src family members such as Yes, Fyn, Fgr and Yrk when phosphorylated at the equivalent sites.

Phospho-Src (Tyr527) Antibody兔多抗能识别内源性Tyr527位点磷酸化的Src蛋白水平。该抗体可能与其他Src家族成员发生交叉反应,如相同位点磷酸化的Yes, Fyn, Fgr 和Yrk 。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr527 of human Src. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体是采用合成的与人源Src蛋白Tyr527位点周围残基相一致的磷酸化肽段免疫动物而产生的。该抗体经蛋白A和肽亲和层析纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells, using Phospho-Src (Tyr527) Antibody (A,B) or v-Src antibody (C,D). The phospho-specificity of the antibody was confirmed by treating the membrane with calf intestinal alkaline phosphatase (CIP) (B,D) after Western transfer.

使用Phospho-Src (Tyr527) Antibody兔多抗 (A,B) 和 v-Src antibody兔多抗 (C,D),对NIH/3T3细胞进行western blot检测。该抗体的磷酸化特异性通过牛小肠磷酸酶(CIP)(B,D)处理转印膜后证实。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing membrane and cytoplasmic localization using Phospho-Src (Tyr527) Antibody.

使用Phospho-Src (Tyr527) Antibody兔多抗,对石蜡包埋的人乳腺癌组织进行免疫组织化学检测,图中显示出胞膜和细胞质的位置。

Background

The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2).

Src家族蛋白酪氨酸激酶包括Src, Lyn, Fyn, Yes, Lck, Blk, Hck, 对调节真核细胞的生长和分化很重要(1)。Src的活性是通过两个酪氨酸位点的磷酸化来调控的,但是这两者的调控效应相反。激酶结构域的活化环中的Tyr416位点的磷酸化,能够上调酶活性;而蛋白羧基端尾部Tyr527位点被Csk磷酸化后,则会使酶的活性降低(2)。

  1. Thomas, S.M. and Brugge, J.S. (1997) Annu Rev Cell Dev Biol 13, 513-609.
  2. Hunter, T. (1987) Cell 49, 1-4.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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