Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-Src Family (Tyr416) Antibody #2101

Fyn   Hck   Lck   Lyn   sc-12958   Tck   Yes   Yrk  

No. Size Price
2101L 300 µl ( 30 western blots ) ¥8,792.00 现货查询 购买询价
2101S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2101 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 60 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Homology

Species predicted to react based on 100% sequence homology: Chicken, Xenopus,

Specificity / Sensitivity

Phospho-Src Family (Tyr416) Antibody detects endogenous levels of Src only when phosphorylated at tyrosine 416. The antibody may cross-react with other Src family members (Lyn, Fyn, Lck, Yes and Hck) when phosphorylated at equivalent sites. It does not cross-react with Src phosphorylated at tyrosine 527. It may cross react with phosphorylated RTKs.

Phospho-Src Family (Tyr416) Antibody兔多抗能够检测内源性的Tyr416位点磷酸化的Src蛋白。该抗体可能会与相同位点被磷酸化的其他Src家族成员(Lyn, Fyn, Lck, Yes和Hck)发生交叉反应。该抗体不会与Tyr527位点被磷酸化的Src蛋白发生交叉反应。可能与磷酸化的RTKs发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr416 of human Src. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体是采用与人源Src蛋白Tyr416位点附近相一致的合成磷酸化肽段免疫动物而产生的。该抗体经蛋白A和肽亲和层析纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from serum-starved COLO 201 cells, v-Src antibody immunoprecipitates from COLO 201 cells and PDGF-treated NIH/3T3 cells, treated with lambda phosphatase as indicated, using Phospho-Src (Tyr416) Antibody (upper) or v-Src antibody (lower).

采用Phospho-Src (Tyr416) Antibody兔多抗 (上图) 和v-Src antibody兔多抗(下图),对血清饥饿的COLO 201细胞提取物及其v-Src antibody兔多抗免疫沉淀物,和用PDGF处理的NIH/3T3细胞提取物的Western blot分析结果。

Background

The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2).

Src家族蛋白酪氨酸激酶包括Src, Lyn, Fyn, Yes, Lck, Blk, Hck, 对调节真核细胞的生长和分化很重要(1)。Src的活性是通过两个酪氨酸位点的磷酸化来调控的,但是这两者的调控效应相反。激酶结构域的活化环中的Tyr416位点的磷酸化,能够上调酶活性;而蛋白羧基端尾部Tyr527位点被Csk磷酸化后,则会使酶的活性降低(2)。

Lyn is a member of the Src family that is predominantly expressed in hematopoietic cells (3). Lyn participates in signaling from multiple cell surface receptors such as the B cell antigen receptor (BCR) and CD40 (4). 
 Lck is essential for T-lymphocyte activation and differentiation (5,6). Phosphorylation of the carboxy-terminal Tyr505 downregulates Lck activity, while phosphorylation at Tyr394 leads to an increase in Lck activity (7).

Lyn是Src家族成员,主要表达于造血细胞中 (3)。Lyn参与了多种细胞表面受体的信号转导,比如B细胞抗原受体(BCR)和CD40 (4)。Lck蛋白对T淋巴细胞的活化和分化非常重要(5,6)。羧端Tyr505位点的磷酸化会下调Lck蛋白活性,而Tyr394位点的磷酸化导致Lck蛋白活性的增高 (7)。

  1. Thomas, S.M. and Brugge, J.S. (1997) Annu Rev Cell Dev Biol 13, 513-609.
  2. Hunter, T. (1987) Cell 49, 1-4.
  3. Yamanshi,, Y. et al. (1989) Proc. Natl. Acad. Sci. USA 86, 6538-6542.
  4. Yamanashi, Y. et al. (1991) Science 251, 192-194.
  5. Molina, T. J. et al. (1992) Nature 357, 161-164.
  6. Straus, D. B. et al. (1992) Cell 70, 585-593.
  7. Chow, L. M. et al. (1993) Nature 365, 156-160.

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