Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

CDK7 Antibody #2090

cyclin  

No. Size Price
2090S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
2090 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 40 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

CDK7 Antibody detects endogenous levels of total CDK7 protein. CDK7 Antibody 能够检测内源性CDK7总蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of human CDK7. Antibodies are purified using protein A and peptide affinity chromatography. 该多克隆抗体是由合成的人源的针对CDK7蛋白氨基末端肽段免疫动物,利用蛋白A和多肽亲和层析技术纯化生产的。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using CDK7 Antibody. Western blot 方法检测不同细胞提取物,使用的抗体为Western blot 。

Background

CDK-activating kinase (CAK) is a complex of CDK7 and cyclin H. The complex is involved in cell cycle regulation by phosphorylating an activating residue in the T-loop domain of cdks (1). Regulation of CAK activity is mediated by T-loop phosphorylation and by association with MAT1, both of which enhance its kinase activity toward the CTD of RNA polymerase II (2,3) and other substrates such as p53 (4). CAK is an essential component of the transcription complex TFIIH and may interact directly with TFIIH helicases (5). CDK激活激酶(CAK)是CDK7和周期蛋白H的复合体。该复合体通过磷酸化cdks的T-回路结构域的激活残基参与细胞周期的调节(1)。CAK活性的调节经由T-回路的磷酸化和MAT1的缔合来介导,这两个过程都能够增强CAK对于RNA 聚合酶II(2,3)的CTD以及其它底物如p53(4)的激酶活性。CAK是转录复合体TFIIH必不可少的组成部分,并可以直接和TFIIH解旋酶相互作用(5)。

  1. Fisher, R.P. et al. (1994) Cell 78, 713-724.
  2. Larochelle, S. et al. (2001) EMBO J. 16, 3749-3759.
  3. Yankulov, K.Y. et al. (1997) EMBO J. 16, 1638-1646.
  4. Ko, L.J. et al. (1997) Mol. Cell. Biol. 17, 7220-7229.
  5. Rossignol, M. et al. (1997) EMBO J. 16, 1628-1637.

Application References

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Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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