Cell Signaling Technology

Product Pathways - Protein Translation

Phospho-Raptor (Ser792) Antibody #2083

792   KIAA1303   P-Raptor   p-Raptor (S792)   p-Raptor(S792)   Phospho-Raptor   raptor   Raptor (S792)   Raptor (Ser972)   Raptor S792   Raptor Ser972   Raptor(S792)   Raptor(Ser972)   Regulatory-associated protein of mTOR   S792   Ser792  

No. Size Price
2083S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2083T 20 µl ( 2 western blots ) ¥1,300.00 现货查询 购买询价
2083 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 150 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-Raptor (Ser792) Antibody detects endogenous levels of raptor protein only when phosphorylated at Ser792. The antibody may also detect non-specific signals of various molecular weights.

Phospho-Raptor (Ser792) Antibody能够检测内源性的Ser792位点磷酸化的raptor蛋白。这个抗体也可能检测到不同分子量的非特异性信号。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to the sequence surrounding Ser792 of human raptor. Antibodies are purified by peptide affinity chromatography.

该多克隆抗体是由合成的人源的针对raptor蛋白Ser792位点磷酸化肽段免疫动物而生产的。

Western Blotting

Western Blotting

Western blot analysis of wild-type (WT) and AMPKα1 and α2 knockout (KO) mouse embryonic fibroblasts (MEFs), untreated or treated with AICAR (2 mM for 1 hour), using Phospho-Raptor (Ser792) Antibody (upper) or Raptor Antibody #4978 (lower). (Image provided by Dr. Reuben Shaw, Salk Institute for Biological Studies).*Cross-reacting bands at 60, 70 and 240 kDa.

Western blot 方法检测野生型(WT)和 AMPKα1及α2 敲除(KO)的小鼠胚胎成纤维细胞(MEFs),使用的抗体是Phospho-Raptor (Ser792) Antibody (上图)和 Raptor Antibody #4978 (下图)。样品是未处理的或用AICAR (2 mM)处理一个小时的。(图片由Dr. Reuben Shaw, Salk Institute for Biological Studies提供)。交叉反应的条带大小为60, 70 和240 kDa。

Western Blotting

Western Blotting

Western blot analysis of C2C12 or 293 cells, untreated or treated with AICAR (0.5 mM for 30 minutes) or oligomycin (0.5 μM for 30 minutes), using Phospho-Raptor (Ser792) Antibody (upper and lower left ) or Raptor Antibody #2280 (upper and lower right).*Cross-reacting bands at 200 kDa.

Western blot 方法检测C2C12 or 293 细胞,使用的抗体是Phospho-Raptor (Ser792) Antibody (上图和左下图) 和 Raptor Antibody #2280 (上图和右下图)。样品是未处理的或用AICAR (0.5 mM)处理半个小时或用oligomycin (0.5 μM)处理半个小时。交叉反应条带大小为200 kDa。

Background

The regulatory associated protein of mTOR (Raptor) was identified as an mTOR binding partner that mediates mTOR signaling to downstream targets (1,2). Raptor binds to mTOR substrates, including 4E-BP1 and p70 S6 kinase, through their TOR signaling (TOS) motifs and is required for mTOR-mediated phosphorylation of these substrates (3,4). Binding of the FKBP12-rapamycin complex to mTOR inhibits the mTOR-raptor interaction, suggesting a mechanism for rapamycin's specific inhibition of mTOR signaling (5). This mTOR-raptor interaction and its regulation by nutrients and/or rapamycin is dependent on a protein called GβL (6). GβL is also part of the rapamycin-insensitive complex between mTOR and rictor (rapamycin-insensitive companion of mTOR), and may mediate rictor-mTOR signaling to downstream targets including PKCα (7). Furthermore, the rictor-mTOR complex has been identified as the previously elusive PDK2 responsible for the phosphorylation of Akt/PKB on Ser473, facilitating phosphorylation of Akt/PKB on Thr308 by PDK1 and required for the full activation of Akt/PKB (8). 
 
 Recently raptor has been identified as a direct substrate of the AMP-activated protein kinase (AMPK) (9). AMPK phosphorylates raptor on Ser722/Ser792 (9). This phosphorylation is essential for inhibition of the raptor-containing mTOR complex 1 (mTORC1) and induces cell cycle arrest when cells are stressed for energy (9). These findings suggest that raptor is a critical switch that correlates cell cycle progression with energy status.

mTOR的调控关联蛋白(Raptor)被认为是mTOR的结合伴侣并能够调节其信号通路下游靶点(1,2)。Raptor通过4E-BP1和p70 S6激酶等mTOR的底物的TOR信号基序与它们结合,为mTOR介导的这些底物的磷酸化所必需(3,4)。FKBP12-rapamycin复合物和mTOR结合可以抑制mTOR与raptor的相互作用,可能是rapamycin抑制mTOR信号的作用机制(5)。mTOR与raptor的相互作用及营养物质和/或rapamycin对它的调控依赖于GβL蛋白(6)。GβL也是mTOR和rictor(mTOR的rapamycin不敏感的组分)之间的rapamycin不敏感复合物的一部分,并可能调节rictor-mTOR信号通路下游像PKCα这些靶点(7)。此外,rictor-mTOR复合物被认为是以前不明了的PDK2,负责Akt / PKB Ser473位点磷酸化,促进PDK1调节的Akt / PKB Thr308位点磷酸化,最终充分激活Akt / PKB(8)。最近raptor被证实是AMP活化的蛋白激酶(AMPK)的直接底物(9)。AMPK磷酸化raptord的Ser722/792(9)。这种磷酸化对于抑制含raptor的mTOR复合物1(mTORC1)的活性并在细胞能量匮乏时诱导细胞周期阻滞是必不可少的(9)。这些结果表明,raptor是调控细胞周期进程和能量状态一个关键节点。

  1. Hara, K. et al. (2002) Cell 110, 177-189.
  2. Kim, D. et al. (2002) Cell 110, 163-175.
  3. Beugnet, A. et al. (2003) J. Biol. Chem. 278, 40717-40722.
  4. Nojima, H. et al. (2003) J. Biol. Chem. 278, 15461-15464.
  5. Oshiro, N. et al. (2004) Genes Cells 9, 359-366.
  6. Kim, D. H. et al. (2003) Mol. Cell 11, 895-904.
  7. Sarbassov, D. et al. (2004) Curr. Biol. 14, 1296-1302.
  8. Sarbassov, D.D. et al. (2005) Science 307, 1098-1101.
  9. Gwinn, D.M. et al. (2008) Mol Cell 30, 214-26.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications