Cell Signaling Technology

Product Pathways - NF-kB Signaling

Phospho-IKKα (Ser176)/IKKβ (Ser177) (C84E11) Rabbit mAb #2078


No. Size Price
2078S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2078T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
2078 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 85 (IKKalpha), 87 (IKKbeta) Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Rat, Monkey, Bovine,

Specificity / Sensitivity

Phospho-IKKα (Ser176)/IKKβ (Ser177) (C84E11) Rabbit mAb detects endogenous levels of IKKα and IKKβ only when phosphorylated at Ser176 and Ser177, respectively.

Phospho-IKKα (Ser176)/IKKβ (Ser177) (C84E11) Rabbit mAb能分别检测内源的在Ser176 和Ser177位点磷酸化的IKKα和IKKβ 蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser177 of IKKβ.

此单克隆抗体是通过合成人源对应的IKKβ Ser177位点周围的肽段来免疫动物而获得。

Western Blotting

Western Blotting

Western blot analysis of extracts from differentiated THP-1 cells, untreated or LPS-treated for 15 minutes, using Phospho-IKKα (Ser176)/IKKβ (Ser177) (C84E11) Rabbit mAb (upper), IKKα Antibody #2682 (middle) and IKKβ (2C8) Rabbit mAb #2370 (lower).Western免疫印迹。用Phospho-IKKα (Ser176)/IKKβ (Ser177) (C84E11) Rabbit mAb (上图), IKKα Antibody #2682 (中间图) 和 IKKβ (2C8) Rabbit mAb #2370 (下图)研究未经处理的和经 LPS处理15 min的已经分化的THP-1细胞的细胞提取液。


The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex, whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation; Ser177 and Ser181 in the activation loop of IKKβ (serine 176 and 180 in IKKα) are the specific sites whose phosphorylation causes conformational changes resulting in kinase activation (10-13).

NF-κB/Rel转录调控因子在细胞质中与IκB抑制蛋白结合以非活性形式存在(1-3)。大部分激活因素采用通常信号通路即磷酸化诱导并通过蛋白水解酶体介导降解IκB的方法激活NF-κB (3-7)。此通路中最重要的步骤是激活高分子量的IκB激酶(IKK)复合体,此复合体催化的反应中有三个紧密相关的IKK亚基参与完成,IKKα and IKKβ 作为激酶的催化单元,IKKγ作为调控单元(8,9)。IKK的激活依赖于特定位点的磷酸化:IKKβ活性环中的 Ser177和Ser181位点以及IKKα中的serine 176 和 180 位点,这些位点的磷酸化引起蛋白构象的变化从而激活激酶(10-13)。

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Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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