Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

Phospho-PKD/PKCμ (Ser744/748) Antibody #2054

P-PKC   PKC-mu   PKD   PKD-PKC   PKD-PKC-mu  

No. Size Price
2054L 300 µl ( 30 western blots ) ¥8,792.00 现货查询 购买询价
2054S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2054T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
2054 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 115 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-PKD/PKCμ (Ser744/748) Antibody detects PKD1/PKCμ only when dually phosphorylated at serines 744 and 748. This antibody may also cross-react with isoforms PKD2 and PKD3/PKCη in some species.

Phospho-PKD/PKCμ (Ser744/748)抗体识别Ser744和Ser748同时磷酸化的PKD/PKCμ。此抗体在某些物种中可能与PKD2和PKD3/PKCη亚型有交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser744/748 of mouse PKD. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells, untreated, PDGF-treated (50 ng/ml) or TPA-treated (0.2 µM), using Phospho-PKD/PKCµ (Ser744/748) Antibody (upper) or PKD/PKCµ Antibody #2052 (lower).

对NIH/3T3细胞(未处理,或50ng/ml PDGF处理,或0.2uM TPA处理),使用Phospho-PKD/PKCµ (Ser744/748)抗体(上图)或PKD/PKCµ抗体#2052(下图)进行Western blot分析。


Activation of PKC is one of the earliest events in a cascade leading to a variety of cellular responses such as secretion, gene expression, proliferation and muscle contraction (1,2). Protein kinase D (PKD), also called PKCμ, is a serine/threonine kinase whose activation is dependent on the phosphorylation of two activation loop sites, Ser744 and Ser748, via a PKC-dependent signaling pathway (3-5). In addition to the two activation loop sites, the carboxy-terminal Ser916 has been identified as an autophosphorylation site for PKD/PKCμ. Phosphorylation at Ser916 correlates with PKD/PKCμ catalytic activity (6).


  1. Nishizuka, Y. (1984) Nature 308, 693-698.
  2. Keranen, L.M. (1995) Curr. Biol. 5, 1394-1403.
  3. Valverde, A.M. et al. (1994) Proc. Natl. Acad. Sci. 91, 8572-8576.
  4. Johannes, F.J. et al. (1994) J. Biol. Chem. 269, 6140-6148.
  5. Iglesias, T. et al. (1998) J. Biol. Chem. 273, 27662-27667.
  6. Matthews, S.A. et al. (1999) J. Biol. Chem. 274, 26543-26549.
  7. Wood, C.D. et al. (2005) J Biol Chem 280, 6245-51.
  8. Amadesi, S. et al. (2009) J Comp Neurol 516, 141-56.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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