Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

Phospho-PKD/PKCμ (Ser916) Antibody #2051

PKC   PKC-mu   PKCmu  

No. Size Price
2051L 300 µl ( 30 western blots ) ¥8,792.00 现货查询 购买询价
2051S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2051T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
2051 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 115 Rabbit
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Phospho-PKD/PKC mu (Ser916) Antibody detects endogenous levels of PKD1/PKC mu only when phosphorylated at serine 916. Ths antibody may also cross-react with isoform PKD2, in some species.

Phospho-PKD/PKC mu (Ser916)抗体识别内源性的Ser916磷酸化的PKD1/PKC mu。此抗体在某些物种中可能与PKD2亚型有交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser916 of mouse PKD. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体用与小鼠PKD中Ser916位点附近的氨基酸序列对应的人工合成磷酸化的肽段免疫动物而制成。该抗体使用蛋白A和蛋白亲和层析纯化而得。

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells, untreated, PDGF-treated (50 ng/ml) or TPA-treated (0.2 µM), using Phospho-PKD/PKCµ (Ser916) Antibody (upper) or PKD/PKCµ Antibody #2052 (lower).

对NIH/3T3细胞(未处理,或50ng/ml PDGF处理,或0.2uM TPA处理),使用Phospho-PKD/PKCµ (Ser916)抗体(上图)或PKD/PKCµ抗体#2052(下图)进行Western blot分析。

Background

Activation of PKC is one of the earliest events in a cascade leading to a variety of cellular responses such as secretion, gene expression, proliferation and muscle contraction (1,2). Protein kinase D (PKD), also called PKCμ, is a serine/threonine kinase whose activation is dependent on the phosphorylation of two activation loop sites, Ser744 and Ser748, via a PKC-dependent signaling pathway (3-5). In addition to the two activation loop sites, the carboxy-terminal Ser916 has been identified as an autophosphorylation site for PKD/PKCμ. Phosphorylation at Ser916 correlates with PKD/PKCμ catalytic activity (6).

PKC的激活是导致多种细胞反应,如分泌、基因表达、细胞增殖和肌肉收缩的级联反应中的最早期事件之一(1,2)。蛋白激酶D(PKD),也被称为PKCμ,是一种丝氨酸/苏氨酸激酶,其激活依赖于两个活性环位点Ser744和Ser748通过PKC依赖的信号通路的磷酸化(3-5)。除了两个活性环位点,羧基端Ser916位点被确定为PKD/PKCμ自身磷酸化的位点。磷酸化Ser916与PKD/PKCμ的催化活性相关(6)。

  1. Nishizuka, Y. (1984) Nature 308, 693-698.
  2. Keranen, L.M. (1995) Curr. Biol. 5, 1394-1403.
  3. Valverde, A.M. et al. (1994) Proc. Natl. Acad. Sci. 91, 8572-8576.
  4. Johannes, F.J. et al. (1994) J. Biol. Chem. 269, 6140-6148.
  5. Iglesias, T. et al. (1998) J. Biol. Chem. 273, 27662-27667.
  6. Matthews, S.A. et al. (1999) J. Biol. Chem. 274, 26543-26549.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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