Cell Signaling Technology

Product Pathways - Apoptosis

Jurkat Apoptosis Cell Lysates (etoposide) #2043

control lysates   etoposide  

No. Size Price
2043S 100 µl ( 10 western blots ) ¥1,450.00 现货查询 购买询价 防伪查询
2043 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:


Total cell lysates from Jurkat cells (a human lymphoma cell line) were untreated or treated with 25 μM etoposide for 5 hours to activate apoptotic cascades and induce proteolytic cleavage of various apoptosis-related proteins including caspases, IAP and PARP. This lysate pair is produced as a molecular weight control for western blotting of various apoptosis-related proteins. Boil lysates for 2 minutes in the original tube, then load 10-15 μl per mini-gel lane. This product will not work with Cleaved Caspase 3 antibodies.

Jurkat 细胞 (一种人类淋巴细胞细胞系) 的全细胞裂解液经未处理或者25 μM etoposide 处理5小时,以激活凋亡信号通路,并诱导各种凋亡相关蛋白的蛋白水解,包括caspases、IAP 和PARP。该裂解液对在 western blotting 免疫印记中作为各种凋亡相关蛋白的分子量对照。在初始管中,将裂解液煮沸2 分钟,然后在mini胶每个上样孔中上样10-15 μl。此产品对 Cleaved Caspase 3 antibodies不工作。


PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).

PARP,是核多聚(ADP-核糖) 聚合酶,分子量116kDa,参与环境胁迫应答中的DNA修复(1)。在体外该蛋白可被许多ICE样 caspases裂解(2,3),在体内是caspase-3裂解的主要靶蛋白之一(4,5)。在人体中PARP在位点Asp214 和 Gly215被裂解,裂解后PARR的N末端DNA 结合结构域(24 kDa)与C端催化结构域(89 kDa) 分离(2,4)。PARP协助细胞维持生存能力;PARP的裂解促进细胞崩解并可以作为细胞凋亡的标记物(6)。

  1. Baker, S.J. and Reddy, E.P. (1998) Oncogene 17, 3261-3270.
  2. Budihardjo, I. et al. (1999) Annu. Rev. Cell Dev. Biol. 15, 269-290.
  3. Nakagawa, T. et al. (2000) Nature 403, 98-103.
  4. Deveraux, Q. L. et al. (1998) EMBO J. 17, 2215-2223.
  5. Li, F. et al. (1998) Nature 396, 580-584.
  6. Du, C. et al. (2000) Cell 102, 33-42.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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