Cell Signaling Technology

Product Pathways - Apoptosis

Cleaved Lamin A (Small Subunit) (30H5) Mouse mAb #2036


No. Size Price
2036S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
2036 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 28 Mouse IgG1
IF-IC 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Cleaved Lamin A (Small Subunit) (30H5) Mouse mAb detects endogenous levels of the small fragment of lamin A (and lamin C) resulting from cleavage at aspartic acid 230. The antibody does not cross-react with full length lamin A or C.

Cleaved Lamin A (Small Subunit) (30H5) Mouse mAb鼠单抗能够检测内源的Asp230位点酶切的lamin A(和C 蛋白)小片段。此抗体不与全长lamins A发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues surrounding Asp230 of human lamin A.

该单克隆抗体是采用合成的人源lamin A/C蛋白C末端Asp230周围残基相对应的肽段免疫动物而制备的。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3, and C6 cells, untreated or staurosporine-treated (1 µM), and Jurkat cells, untreated or etoposide-treated (25 µM), using Cleaved Lamin A (Small Subunit) (30H5) Mouse mAb.

Western blot 检测未处理或staurosporine(星形孢菌素)(1 µM)处理的HeLa、NIH/3T3和C6细胞提取物,以及未处理或etoposide(依托泊甙)处理的 Jurkat 细胞,使用抗体为 Cleaved Lamin A (Small Subunit) (30H5) Mouse mAb鼠单抗。



Immunofluorescent analysis of HeLa cells, untreated (right) or staurosporine-treated (left), using Cleaved Lamin A (Small Subunit) (30H5) Mouse mAb.

Western blot检测HeLa细胞提取物,未处理(右)或staurosporine(星形孢菌素)(左)处理,使用抗体为 Cleaved Lamin A (Small Subunit) (30H5) Mouse mAb鼠单抗。


Lamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control, DNA replication and chromatin organization (1-3). Lamin A/C is cleaved by caspase-6 and serves as a marker for caspase-6 activation. During apoptosis, lamin A/C is specifically cleaved into a large (41-50 kDa) and a small (28 kDa) fragment (3,4). The cleavage of lamins results in nuclear disregulation and cell death (5,6).

Lamin是核膜的结构组分,对维持正常的细胞功能非常重要,比如细胞周期调控、DNA修复和染色质组装(1-3)。Lamin A/C能够被caspase-6切割,可以作为caspase-6激活的标志。在凋亡过程中,Lamin A/C能够被特异地剪切成大片段(41-50 kDa)和小片段 (28 kDa)(3,4)。Lamin的剪切会引起细胞核的失调和细胞死亡(5,6)。

  1. Gruenbaum, Y. et al. (2000) J Struct Biol 129, 313-23.
  2. Yabuki, M. et al. (1999) Physiol Chem Phys Med NMR 31, 77-84.
  3. Goldberg, M. et al. (1999) Crit Rev Eukaryot Gene Expr 9, 285-93.
  4. Orth, K. et al. (1996) J Biol Chem 271, 16443-6.
  5. Oberhammer, F.A. et al. (1994) J Cell Biol 126, 827-37.
  6. Rao, L. et al. (1996) J Cell Biol 135, 1441-55.

Application References

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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