Cell Signaling Technology

Product Pathways - Apoptosis

Cleaved Lamin A (Small Subunit) Antibody #2035


No. Size Price
2035S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
2035 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 28 Rabbit
IHC-P 1:100
IF-IC 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Cleaved Lamin A (Small Subunit) Antibody detects the small fragment of lamin A (and lamin C) resulting from cleavage at aspartic acid 230 by caspase-6. The antibody does not cross-react with full length lamin A or lamin C.

Cleaved Lamin A (Small Subunit) Antibody能够检测在Asp230位点被caspase-6酶切的lamin A (和lamin C)蛋白小片段。该抗体不与全长lamin A或lamin C发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues surrounding Asp230 in human lamin A. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体是采用合成的人源lamin A/C蛋白N末端Asp230周围残基相对应的肽段免疫动物而制备的。体由protein A和肽亲和层析技术纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3 or C6 cells, untreated or staurosporine-treated (1 µM), using Cleaved Lamin A (Small Subunit) Antibody.

Western blot 检测HeLa、NIH/3T3或C6 细胞提取物,未处理或staurosporine(星形孢菌素)(1 µM)处理,使用抗体为Cleaved Lamin A (Small Subunit)。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human tonsil using Cleaved Lamin A (Small Subunit) Antibody.

免疫组织化学分析石蜡包埋的人扁桃体切片,使用抗体为 Cleaved Lamin A (Small Subunit) Antibody 。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis using Cleaved Lamin A (Small Subunit) Antibody on SignalSlide® Cleaved Caspase-3 (Asp175) IHC Controls #8104 (Jurkat cell pellets,untreated (left) or etopside treated (right).

使用 SignalSlide® Cleaved Caspase-3 (Asp175) IHC Controls #8104 和Cleaved Lamin A (Small Subunit) Antibody进行免疫组织化学分析。(Jurkat细胞粒,未处理(左)或etopside处理(右))



Confocal immunofluorescent analysis of HeLa cells, untreated (left) or staurosporine-treated (right), using Cleaved Lamin A (Small Subunit) Antibody (green). Actin filaments have been labeled using DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

共聚焦免疫荧光分析HeLa细胞,未处理(左)和Staurosporine(右)处理,使用抗体为 Cleaved Lamin A (Small Subunit) Antibody(绿色)。DY-554 phalloidin(红色)标记肌动蛋白丝。蓝色伪彩=DRAQ5®#4084(荧光DNA染料)。


Lamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control, DNA replication and chromatin organization (1-3). Lamin A/C is cleaved by caspase-6 and serves as a marker for caspase-6 activation. During apoptosis, lamin A/C is specifically cleaved into a large (41-50 kDa) and a small (28 kDa) fragment (3,4). The cleavage of lamins results in nuclear disregulation and cell death (5,6).

Lamin是核膜的结构组分,对维持正常的细胞功能非常重要,比如细胞周期调控、DNA修复和染色质组装(1-3)。Lamin A/C能够被caspase-6切割,可以作为caspase-6激活的标志。在凋亡过程中,Lamin A/C能够被特异地剪切成大片段(41-50 kDa)和小片段 (28 kDa)(3,4)。Lamin的剪切会引起细胞核的失调和细胞死亡(5,6)。

  1. Gruenbaum, Y. et al. (2000) J Struct Biol 129, 313-23.
  2. Yabuki, M. et al. (1999) Physiol Chem Phys Med NMR 31, 77-84.
  3. Goldberg, M. et al. (1999) Crit Rev Eukaryot Gene Expr 9, 285-93.
  4. Orth, K. et al. (1996) J Biol Chem 271, 16443-6.
  5. Oberhammer, F.A. et al. (1994) J Cell Biol 126, 827-37.
  6. Rao, L. et al. (1996) J Cell Biol 135, 1441-55.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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