Cell Signaling Technology

Product Pathways - Apoptosis

Cleaved Lamin A (Asp230) Antibody #2031

Lamin-A  

No. Size Price
2031S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2031 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 45, 50 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Cleaved Lamin A (Asp230) Antibody detects endogenous levels of the large fragment of lamin A (and lamin C isotype) resulting from cleavage at aspartic acid 230. The antibody does not recognize full length lamin A.

Cleaved Lamin A (Asp230) Antibody 能够检测内源的Asp230位点酶切的lamin A(和C 蛋白)的大片段。此抗体不与全长lamins A发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to carboxy-terminal residues surrounding Asp230 in human lamin A. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体是采用合成的人源lamin A/C蛋白C末端Asp230周围残基相对应的肽段免疫动物而制备的。体由protein A和肽亲和层析技术纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated or staurosporine-treated (1 µM), using Cleaved Lamin A (Asp230) Antibody (upper) or Lamin A Antibody #2032 (lower).

Western blot检测HeLa细胞提取物,未处理或staurosporine(星形孢菌素)(1 µM)处理,使用抗体为Cleaved Lamin A (Asp230) Antibody (上图)或Lamin A Antibody #2032 (下图)。

Background

Lamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control, DNA replication and chromatin organization (1-3). Lamin A/C is cleaved by caspase-6 and serves as a marker for caspase-6 activation. During apoptosis, lamin A/C is specifically cleaved into a large (41-50 kDa) and a small (28 kDa) fragment (3,4). The cleavage of lamins results in nuclear disregulation and cell death (5,6).

Lamin是核膜的结构组分,对维持正常的细胞功能非常重要,比如细胞周期调控、DNA修复和染色质组装(1-3)。Lamin A/C能够被caspase-6切割,可以作为caspase-6激活的标志。在凋亡过程中,Lamin A/C能够被特异地剪切成大片段(41-50 kDa)和小片段 (28 kDa)(3,4)。Lamin的剪切会引起细胞核的失调和细胞死亡(5,6)。

(This product is sold under license from Promega Corp., U.S. Patent No. 6,350,452.) (此产品经Promega公司许可销售,美国专利号是第6350452号)。

  1. Gruenbaum, Y. et al. (2000) J Struct Biol 129, 313-23.
  2. Yabuki, M. et al. (1999) Physiol Chem Phys Med NMR 31, 77-84.
  3. Goldberg, M. et al. (1999) Crit Rev Eukaryot Gene Expr 9, 285-93.
  4. Orth, K. et al. (1996) J Biol Chem 271, 16443-6.
  5. Oberhammer, F.A. et al. (1994) J Cell Biol 126, 827-37.
  6. Rao, L. et al. (1996) J Cell Biol 135, 1441-55.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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