Cell Signaling Technology

Product Pathways - Apoptosis

Phospho-Lamin A/C (Ser22) Antibody #2026

70 kDa lamin   lamina/c   laminc   LMN1   LMNA  

No. Size Price
2026S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2026 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 69, 78 Rabbit
IF-IC 1:800

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Phospho-Lamin A/C (Ser22) Antibody detects endogenous levels of lamin A/C only when phosphorylated at Ser22.

Phospho-Lamin A/C (Ser22)抗体只能用于检测内源性的Ser32磷酸化的lamin A/C蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser22 of human lamin A/C protein.

该多克隆抗体是采用合成的人源lamin A/C蛋白Ser22 周围残基相对应的肽段免疫动物而制备的。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa and NIH/3T3 cells, hydroxyurea-treated (4 mM, 20 hours) to induce G1/S phase or paclitaxel-treated (100 nM, 20 hours) or nocodazole-treated (100 ng/ml, 20 hours) to induce G2/M phase, using Phospho-Lamin A/C (Ser22) Antibody (upper) or Lamin A/C Antibody #2032 (lower) as a loading control.

免疫印迹检测HeLa细胞和NIH/3T3细胞的细胞提取物,用hydroxyurea(羟基脲)(4 mM, 20 小时)处理诱导G1/S期,用paclitaxel(紫杉醇)(100 nM, 20 小时)或nocodazole(诺考达唑 (100 ng/ml, 20 h小时)处理诱导G2/M期:使用Phospho-Lamin A/C (Ser22) Antibody (上) 或 Lamin A/C Antibody #2032 (下) 作为上样对照。

Western Blotting

Western Blotting

Western blot analysis of extract from G2/M phase HeLa cells untreated or treated with phosphatase, using Phospho-Lamin A/C (Ser22) Antibody (upper) or Lamin A/C Antibody (lower) as loading control.

免疫印迹分析G2/M期HeLa细胞未经过或经过磷酸酶处理的细胞提取物,使用Phospho-Lamin A/C (Ser22) Antibody (上) or Lamin A/C Antibody (下) 作为上样对照。

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using Phospho-Lamin A/C (Ser22) Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

免疫荧光实验分析HeLa细胞,使用Phospho-Lamin A/C (Ser22) Antibody (绿)。肌动蛋白微丝用DY-554鬼笔环肽(红)标记。Blue pseudocolor = DRAQ5™ (荧光DNA dye).

Background

Lamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control, DNA replication and chromatin organization (1-3). Lamin A/C is cleaved by caspase-6 and serves as a marker for caspase-6 activation. During apoptosis, lamin A/C is specifically cleaved into a large (41-50 kDa) and a small (28 kDa) fragment (3,4). The cleavage of lamins results in nuclear disregulation and cell death (5,6).

Lamin是核膜的结构组分,对维持正常的细胞功能非常重要,比如细胞周期调控、DNA修复和染色质组装(1-3)。Lamin A/C能够被caspase-6切割,可以作为caspase-6激活的标志。在凋亡过程中,Lamin A/C能够被特异地剪切成大片段(41-50 kDa)和小片段 (28 kDa)(3,4)。Lamin的剪切会引起细胞核的失调和细胞死亡(5,6)。

Phosphorylation of Lamin A/C at Ser22 was identified in vivo in several cell lines by mass spectrometry analysis in proteomic screens. The surrounding sequence is a typical MAPK/CDK phosphorylation motif, which implicates a role in the cell cycle and mitosis (7-11).

用质谱分析技术研究蛋白质组学时发现,多种细胞系中都存在Lamin A/C的Ser22磷酸化。其周围的序列是典型的MAPK/CDK磷酸化基序,这表明它在细胞周期和有丝分裂中发挥作用(7-11)。

  1. Gruenbaum, Y. et al. (2000) J Struct Biol 129, 313-23.
  2. Yabuki, M. et al. (1999) Physiol Chem Phys Med NMR 31, 77-84.
  3. Goldberg, M. et al. (1999) Crit Rev Eukaryot Gene Expr 9, 285-93.
  4. Orth, K. et al. (1996) J Biol Chem 271, 16443-6.
  5. Oberhammer, F.A. et al. (1994) J Cell Biol 126, 827-37.
  6. Rao, L. et al. (1996) J Cell Biol 135, 1441-55.
  7. Lowery, D.M. et al. (2007) EMBO J 26, 2262-73.
  8. Molina, H. et al. (2007) Proc Natl Acad Sci USA 104, 2199-204.
  9. Beausoleil, S.A. et al. (2006) Nat Biotechnol 24, 1285-92.
  10. Nousiainen, M. et al. (2006) Proc Natl Acad Sci USA 103, 5391-6.
  11. Beausoleil, S.A. et al. (2004) Proc Natl Acad Sci USA 101, 12130-5.

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For Research Use Only. Not For Use In Diagnostic Procedures.

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