Cell Signaling Technology

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Phospho-β-Catenin (Ser33/37) Antibody #2009

catenin  

No. Size Price
2009S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2009 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 92 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat, Xenopus, Zebrafish,

Specificity / Sensitivity

Phospho-β-Catenin (Ser33/37) Antibody detects endogenous levels of β-catenin only when phosphorylated at Ser33 and Ser37.

磷酸化β-Catenin(Ser33/37)抗体仅识别33位丝氨酸和37位丝氨酸磷酸化的内源性β-Catenin蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser33 and Ser37 of human β-catenin. Antibodies are purified by protein A and peptide affinity chromatography.

此多克隆抗体由合成磷酸化肽段免疫动物产生,合成的肽段与人β-catenin的33位丝氨酸和37位丝氨酸邻近氨基酸残基序列一致,抗体由蛋白A和肽段亲和层析技术纯化.

Western Blotting

Western Blotting

Western blot analysis of extracts from SW620 cells, untreated or treated with Calyculin A #9902, using Phospho-β-Catenin (Ser33/37) Antibody.

使用Phospho-β-Catenin (Ser33/37)抗体对未处理或Calyculin A#9902处理的SW620细胞提取物进行western blot分析。

Western Blotting

Western Blotting

Western blot analysis of extracts from SW620 cells, untreated or treated with λ phosphatase, using Phospho-β-Catenin (Ser33/37) Antibody.

使用Phospho-β-Catenin (Ser33/37) 抗体对未处理或经过λ 磷酸酯酶处理的SW620细胞提取物进行western blot分析。

Background

β-Catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).

β-catenin是Wnt信号通路下游的重要效应分子(1)。在脊椎动物体内它涉及到两个重要的生物过程:早期胚胎发育(2)和肿瘤发生(3)。CK1可以磷酸化β-catenin 45位丝氨酸。该磷酸化将导致β-catenin能够随后被GSK-3磷酸化(4-6)。GSK-3β通过磷酸化β-catenin Ser33, Ser37, 和 Thr41,并促使其进一步被降解(7)。这些位点发生突变会提高β-catenin蛋白稳定性,并且已在多种癌细胞株中发现这些突变(8)。

  1. Cadigan, K.M. and Nusse, R. (1997) Genes Dev 11, 3286-305.
  2. Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88.
  3. Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21.
  4. Amit, S. et al. (2002) Genes Dev 16, 1066-76.
  5. Liu, C. et al. (2002) Cell 108, 837-47.
  6. Yanagawa, S. et al. (2002) EMBO J 21, 1733-42.
  7. Yost, C. et al. (1996) Genes Dev 10, 1443-54.
  8. Morin, P.J. et al. (1997) Science 275, 1787-90.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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