Product Pathways - Chromatin IP
Fra2 (D2F1E) Rabbit mAb #19967
|19967S||100 µl ( 10 western blots )||￥3,100.00 现货查询||购买询价|
|19967||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), ChIP=Chromatin IP,
Species predicted to react based on 100% sequence homology: Bovine, Horse,
Specificity / Sensitivity
Fra2 (D2F1E) Rabbit mAb recognizes endogenous levels of total Fra2 protein. This antibody does not cross-react with c-Fos or Fra1.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val245 of human Fra2 protein.
Immunohistochemical analysis of paraffin-embedded T-47D (Fra2 low expression, left) and U-2 OS (Fra2 high expression, right) cell pellets using Fra2 (D2F1E) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Fra2 (D2F1E) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Fra2 (D2F1E) Rabbit mAb.
Western blot analysis of extracts from serum-starved HeLa cells, untreated (-) or stimulated with TPA #4174 (+) for 4 hr, using Fra2 (D2F1E) Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 Jurkat cells and either 10 μl of Fra2 (D2F1E) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human CCR4 promoter primers, SimpleChIP® Human Sox4 Promoter Primers #32943, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
The Fos family of nuclear oncogenes includes c-Fos, FosB, Fos-related antigen 1 (FRA1), and Fos-related antigen 2 (FRA2) (1). While most Fos proteins exist as a single isoform, the FosB protein exists as two isoforms: full-length FosB and a shorter form, FosB2 (Delta FosB), which lacks the carboxy-terminal 101 amino acids (1-3). The expression of Fos proteins is rapidly and transiently induced by a variety of extracellular stimuli including growth factors, cytokines, neurotransmitters, polypeptide hormones, and stress. Fos proteins dimerize with Jun proteins (c-Jun, JunB, and JunD) to form Activator Protein-1 (AP-1), a transcription factor that binds to TRE/AP-1 elements and activates transcription. Fos and Jun proteins contain the leucine-zipper motif that mediates dimerization and an adjacent basic domain that binds to DNA. The various Fos/Jun heterodimers differ in their ability to transactivate AP-1 dependent genes. In addition to increased expression, phosphorylation of Fos proteins by Erk kinases in response to extracellular stimuli may further increase transcriptional activity (4-6). Phosphorylation of c-Fos at Ser32 and Thr232 by Erk5 increases protein stability and nuclear localization (5). Phosphorylation of FRA1 at Ser252 and Ser265 by Erk1/2 increases protein stability and leads to overexpression of FRA1 in cancer cells (6). Following growth factor stimulation, expression of FosB and c-Fos in quiescent fibroblasts is immediate, but very short-lived, with protein levels dissipating after several hours (7). FRA1 and FRA2 expression persists longer, and appreciable levels can be detected in asynchronously growing cells (8). Deregulated expression of c-Fos, FosB, or FRA2 can result in neoplastic cellular transformation; however, Delta FosB lacks the ability to transform cells (2,3).
- Tulchinsky, E. (2000) Histol Histopathol 15, 921-8.
- Dobrazanski, P. et al. (1991) Mol Cell Biol 11, 5470-8.
- Nakabeppu, Y. and Nathans, D. (1991) Cell 64, 751-9.
- Rosenberger, S.F. et al. (1999) J Biol Chem 274, 1124-30.
- Sasaki, T. et al. (2006) Mol Cell 24, 63-75.
- Basbous, J. et al. (2007) Mol Cell Biol 27, 3936-50.
- Kovary, K. and Bravo, R. (1991) Mol Cell Biol 11, 2451-9.
- Kovary, K. and Bravo, R. (1992) Mol Cell Biol 12, 5015-23.
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
SignalStain is a trademark of Cell Signaling Technology, Inc.
SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.
Tween is a registered trademark of ICI Americas, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
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