Cell Signaling Technology

Product Pathways - Development

Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP® Rabbit mAb #19807

No. Size Price
19807S 100 µl ( 10 western blots ) ¥3,580.00 现货查询 购买询价
19807T 20 µl ( 2 western blots ) ¥1,400.00 现货查询 购买询价
19807 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey,Zebrafish,Dog, Endogenous 92 Rabbit IgG
IP 1:50
IHC-P 1:1000
F 1:200
IF-F 1:1600
IF-IC 1:1600

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry, IF-F=Immunofluorescence (Frozen), IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP® Rabbit mAb recognizes endogenous levels of β-catenin protein only when Ser45 is not phosphorylated.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser45 of human β-catenin protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

IF-IC

IF-IC

Confocal immunofluorescent analysis of SW480 (left and lower right; positive) or NCI-H28 (upper right; negative) cells using Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP® Rabbit mAb (green) in the presence of phospho-β-Catenin (Ser45) peptide (lower left) or non-phospho β-Catenin peptide (lower right). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IF-F

IF-F

Confocal immunofluorescent analysis of mouse colon using Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IP

IP

Immunoprecipitation of non-phospho β-catenin (Ser45) protein from COLO 205 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP® Rabbit mAb. Western blot analysis was performed using Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded Apc (Min/+) mouse intestinal adenoma using Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP® Rabbit mAb in the presence of phospho-β-Catenin (Ser45) peptide (left) or non-phospho β-Catenin (Ser45) peptide (right).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded HeLa (left) or NCI-H28 (right) cell pellets using Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP® Rabbit mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of SW480 (green) and NCI-H28 (blue) cells using Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Background

β-Catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).

Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP® Rabbit mAb specifically recognizes β-catenin that is non-phosphorylated at Ser45. Non-phospho β-Catenin (Ser45) protein is useful as readout of stabilized β-catenin protein, and therefore functionally active in cell-cell adhesion and mediating transcriptional activity via the canonical Wnt signaling pathway (9).

  1. Cadigan, K.M. and Nusse, R. (1997) Genes Dev 11, 3286-305.
  2. Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88.
  3. Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21.
  4. Amit, S. et al. (2002) Genes Dev 16, 1066-76.
  5. Liu, C. et al. (2002) Cell 108, 837-47.
  6. Yanagawa, S. et al. (2002) EMBO J 21, 1733-42.
  7. Yost, C. et al. (1996) Genes Dev 10, 1443-54.
  8. Morin, P.J. et al. (1997) Science 275, 1787-90.
  9. Sakanaka, C. (2002) J Biochem 132, 697-703.

Application References

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Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

SignalStain is a trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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