Cell Signaling Technology

Product Pathways - MAPK Signaling

MEKK2 Antibody #19607

MAP3K   MAP3K2   MAPKKK2   MEK kinase 2   MEKK2   MEKK2B  

No. Size Price
19607S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
19607 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 78 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

MEKK2 Antibody recognizes endogenous levels of total MEKK2 protein. This antibody does not cross-react with MEKK3 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly292 of human MEKK2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using MEKK2 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human MEKK2 protein (hMEKK2-Myc/DDK; +), using MEKK2 Antibody.

IP

IP

Immunoprecipitation of MEKK2 from HeLa cell extracts. Lane 1 is 10% input, lane 2 is immunoprecipitation with Normal Rabbit IgG #2729, and lane 3 is immunoprecipitation with MEKK2 Antibody. Western blot analysis was performed using MEKK2 Antibody.

Background

Mitogen-activated protein kinase kinase kinase 2 (MEKK2/MAP3K2) belongs to the MAP3K family of Ser/Thr kinases. Research studies have demonstrated that MEKK2 plays a pivotal role in transducing mitogenic signals emanating from EGFR and FGF2R to JNK and ERK5 signaling cascades (1,2). Post-translationally MEKK2 is regulated through multiple mechanisms including: dimerization (3,4), ubiquitination (5,6), phosphorylation (7) and methylation (8). Research studies implicate dysregulation of MEKK2 signaling in breast carcinoma (9), colorectal carcinoma (10), and pancreatic ductal adenocarcinoma (8).

  1. Sun, W. et al. (2003) Mol Cell Biol 23, 2298-308.
  2. Kesavan, K. et al. (2004) J Cell Physiol 199, 140-8.
  3. Cheng, J. et al. (2005) Mol Cell Biol 25, 5955-64.
  4. Cheng, J. et al. (2005) J Biol Chem 280, 13477-82.
  5. Takeda, A.N. et al. (2014) EMBO J 33, 1784-801.
  6. Yamashita, M. et al. (2005) Cell 121, 101-13.
  7. Zhang, D. et al. (2006) EMBO J 25, 97-107.
  8. Mazur, P.K. et al. (2014) Nature 510, 283-7.
  9. Mirza, A.A. et al. (2014) Biochim Biophys Acta 1843, 945-54.
  10. Jiang, L. et al. (2013) Oncol Lett 6, 1333-37.

Application References

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Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

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