Cell Signaling Technology

Product Pathways - Protein Folding

PCSK7 (D4I5G) Rabbit mAb #19346

No. Size Price
19346S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
19346 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 92 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

PCSK7 (D4I5G) Rabbit mAb recognizes endogenous levels of total PCSK7 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu630 of human PCSK7 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using PCSK7 (D4I5G) Rabbit mAb.

IP

IP

Immunoprecipitation of PCSK7 protein from HT-29 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is PCSK7 (D4I5G) Rabbit mAb. Western blot analysis was performed using PCSK7 (D4I5G) Rabbit mAb.

Background

PCSK7 (PC7) is a member of the subtilisin-like proprotein convertase family (1,2). Like other members of the family, the protein cleaves precursors at basic amino acids within the motif Arg/Lys-Xn-Arg (cleavage site) (n=2 or 4). PC7 was reported to be localized in the trans-golgi network and at the cell surface membrane, as well as in membrane internalized recycling vesicles (2,3). One function of PCSK7 is its critical role in growth factors processing, for example proEGF to EGF, proVEGF-C to VEGF-C, and BDNF neuropeptide maturation. PCSK7 is also involved in transferrin receptor shedding to regulate ion homeostasis (7,8) and MHC class I stability to regulate antigen presentation in the immunoresponse process (9).

  1. Seidah, N.G. and Prat, A. (2012) Nat Rev Drug Discov 11, 367-83.
  2. Rousselet, E. et al. (2011) J Biol Chem 286, 2728-38.
  3. Declercq, J. et al. (2012) J Biol Chem 287, 9052-60.
  4. Rousselet, E. et al. (2011) J Biol Chem 286, 9185-95.
  5. Siegfried, G. et al. (2003) J Clin Invest 111, 1723-32.
  6. Wetsel, W.C. et al. (2013) Proc Natl Acad Sci U S A 110, 17362-7.
  7. Oexle, K. et al. (2011) Hum Mol Genet 20, 1042-7.
  8. Guillemot, J. et al. (2013) Hepatology 57, 2514-24.
  9. Leonhardt, R.M. et al. (2010) J Immunol 184, 2985-98.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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