Cell Signaling Technology

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ST14/Matriptase Antibody #19271

No. Size Price
19271S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
19271 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 80, 95 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

ST14/Matriptase Antibody recognizes endogenous levels of total ST14/Matriptase protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn280 of human ST14/Matriptase protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HCT 116, ZR-75-1, and DLD-1 cells using ST14/Matriptase Antibody.

Background

Suppressor of tumorigenicity 14 protein (ST14), also known as matriptase, is a type II transmembrane protease (1,2). It is highly expressed in epithelia cells and important for the differentiation and homeostasis of epithelium (3). ST14 is synthesized as a single-chain precursor, and cleaved first at Gly149 in endoplasmic reticulum or Golgi. The resulting N-terminal and C-terminal fragments are non-covalently associated, and interact with hepatocyte growth factor activator inhibitor 1 (HAI-1), which facilitates the transportation of ST14 to the plasma membrane. Cell surface ST14 can be activated by autocleavage at Arg614 though an incompletely understood mechanism. HAI-1 inhibits activated ST14, and HAI-1 and ST14 complex can be shed from cell surface (4,5). In different context, ST14 has been reported either as a tumor suppressor or as a tumor promoter (6-8)

  1. Kim, M.G. et al. (1999) Immunogenetics 49, 420-8.
  2. Lin, C.Y. et al. (1999) J Biol Chem 274, 18231-6.
  3. Oberst, M.D. et al. (2003) J Histochem Cytochem 51, 1017-25.
  4. List, K. et al. Mol Med 12, 1-7.
  5. Bugge, T.H. et al. (2007) Front Biosci 12, 5060-70.
  6. Kosa, P. et al. (2012) Oncogene 31, 3679-95.
  7. Sales, K.U. et al. (2015) Oncogene 34, 4664-72.
  8. Ko, C.J. et al. (2015) Cancer Res 75, 2949-60.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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