Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

VISTA (D1L2G™) XP® Rabbit mAb (PE Conjugate) #18946

No. Size Price
18946S 100 µl ( 50 tests ) ¥4,264.00 现货查询 购买询价
18946 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
F 1:50 Human,Monkey, Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry,

Specificity / Sensitivity

VISTA (D1L2G) XP® Rabbit mAb (PE Conjugate) recognizes endogenous levels of total VISTA protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human VISTA protein.

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated VISTA (D1L2G) XP® Rabbit mAb #64953.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of MCF7 cells (blue) and OVKATE cells (green) using VISTA (D1L2G) XP® Rabbit mAb (PE Conjugate).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of human whole blood cells stained with VISTA (D1L2G) XP® Rabbit mAb (PE Conjugate) and co-stained with CD19-Alexa Fluor® 488 and CD14-APC. VISTA is present on CD14+ monocytes (right) and shows a significant increase in intensity when compared to its isotype control (center). CD19+ B cells are negative for VISTA (left).

Background

VISTA (V-Domain Ig Suppressor of T Cell Activation) is a negative checkpoint control protein that regulates T cell activation and immune responses. VISTA, which contains a single Ig-like V-type domain, a transmembrane domain, and an intracellular domain, has sequence similarity to both the B7 and CD28 family members. Although primarily expressed by myeloid cells, VISTA is also expressed by CD4+, CD8+, and FoxP3+ T-cells. Thus, VISTA is described as both a ligand and a receptor (1-3). Blocking VISTA induces T-cell activation and proliferation, and potentiates disease severity in the EAE model (1). Furthermore, genetic deletion of VISTA in mice leads to spontaneous T-cell activation and chronic inflammation (4,5). In mouse models of cancer, neutralization of VISTA enhances T-cell proliferation and effector function and increases tumor infiltration, suggesting VISTA blockade could be an effective strategy for tumor immunotherapy (6,7).

  1. Wang, L. et al. (2011) J Exp Med 208, 577-92.
  2. Flies, D.B. et al. (2011) J Immunol 187, 1537-41.
  3. Lines, J.L. et al. (2014) Cancer Res 74, 1924-32.
  4. Wang, L. et al. (2014) Proc Natl Acad Sci U S A 111, 14846-51.
  5. Liu, J. et al. (2015) Proc Natl Acad Sci U S A 112, 6682-7.
  6. Le Mercier, I. et al. (2014) Cancer Res 74, 1933-44.
  7. Lines, J.L. et al. (2014) Cancer Immunol Res 2, 510-7.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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