Cell Signaling Technology

Product Pathways - Metabolism

HSL (D6W5S) XP® Rabbit mAb #18381

Hormone-sensitive lipase   hormone-sensitive lipase testicular isoform   HSL   LHS   lipase. hormone-sensitive   LIPE   LIPS  

No. Size Price
18381S 100 µl ( 10 western blots ) ¥3,580.00 现货查询 购买询价
18381 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 81, 83 Rabbit IgG
IP 1:100
IF-IC 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

HSL (D6W5S) XP® Rabbit mAb recognizes endogenous levels of total HSL protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His946 of human HSL protein.

IF-IC

IF-IC

Confocal immunofluorescent analysis of differentiated human adipocytes (left) or human pre-adipocytes (right) using HSL (D6W5S) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight® 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of extracts from human pre-adipocytes and differentiated human adipocytes using HSL (D6W5S) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

IP

IP

Immunoprecipitation of HSL from differentiated human adipocyte extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is HSL (D6W5S) XP® Rabbit mAb. Western blot analysis was performed using

HSL (D6W5S) XP® Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from undifferentiated and differentiated 3T3-L1 cells using HSL (D6W5S) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Background

HSL (hormone-sensitive lipase) catalyzes the hydrolysis of triacylglycerol, the rate-limiting step in lipolysis. Lipolytic stimuli activate adenylyl cyclase and thus increase intracellular cAMP levels, which in turn activate protein kinase A (PKA). PKA phosphorylates HSL at Ser563, Ser659, and Ser660, which stimulates HSL activity (1,2). In contrast, AMPK phosphorylates HSL at Ser565, which reduces HSL phosphorylation at Ser563 by PKA and inhibits HSL activity (2,3). Recent work indicates that phosphorylation at Ser600 by p44/42 MAPKs also enhances the enzymatic activity of HSL (4).

  1. Degerman, E. et al. (1990) Proc. Natl. Acad. Sci. USA 87, 533-37.
  2. Anthonsen, M.W. et al. (1998) J. Biol. Chem. 273, 215-21.
  3. Garton, A.J. and Yeaman, S.J. (1990) Eur. J. Biochem. 191, 245-50.
  4. Greenberg, A.S. et al. (2001) J. Biol. Chem. 276, 45456-61.

Application References

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Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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