Cell Signaling Technology

Product Pathways - Neuroscience

Nav1.1 (D8X1Y) Rabbit mAb #18339

No. Size Price
18339S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
18339 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 250 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Nav1.1 (D8X1Y) Rabbit mAb recognizes endogenous levels of total Nav1.1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg471 of human Nav1.1 protein.

Western Blotting

Western Blotting

Western blot analysis of membrane extracts from human, mouse and rat brain using Nav1.1 (D8X1Y) Rabbit mAb.



Immunoprecipitation of Nav1.1 from mouse brain extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Nav1.1 (D8X1Y) Rabbit mAb. Western blot analysis was performed using Nav1.1 (D8X1Y) Rabbit mAb.


Voltage gated sodium channels are composed of a large alpha subunit and auxiliary beta subunits. The alpha subunit has 4 homologous domains, with each domain containing 6 transmembrane segments. These segments function as the voltage sensor and sodium permeable pore. Upon change of membrane potential, the sodium channel is activated, which allows sodium ions to flow through (1,2). When associated with beta subunits or other accessory proteins, the alpha subunit is regulated at the level of cell surface expression, kinetics, and voltage dependence (3,4).

There are 9 mammalian alpha subunits, named Nav1.1-Nav1.9 (5). These alpha subunits differ in tissue specificity and biophysical functions (6,7). Seven of these subunits are essential for the initiation and propagation of action potentials in the central and peripheral nervous system while Nav1.4 and Nav1.5 are mainly expressed in skeletal muscle and cardiac muscle (8,9). Mutations in these alpha channel subunits have been identified in patients with epilepsy, seizure, ataxia, sensitivity to pain, and cardiomyopathy (reviewed in 10).

Nav1.1 is a type I alpha subunit and is mainly expressed in brain and peripheral nerves. Mutations in the Nav1.1 encoding gene, SCN1A, are associated with a wide spectrum of childhood epilepsies, including generalized epilepsy with febrile seizures plus (11) and Dravet syndrome (12,13)

  1. Catterall, W.A. (2000) Neuron 26, 13-25.
  2. Yu, F.H. and Catterall, W.A. (2003) Genome Biol 4, 207.
  3. Isom, L.L. et al. (1994) Neuron 12, 1183-94.
  4. Yu, F.H. et al. (2003) J Neurosci 23, 7577-85.
  5. Goldin, A.L. et al. (2000) Neuron 28, 365-8.
  6. Plummer, N.W. and Meisler, M.H. (1999) Genomics 57, 323-31.
  7. Goldin, A.L. (2001) Annu Rev Physiol 63, 871-94.
  8. George, A.L. et al. (1992) Ann Neurol 31, 131-7.
  9. Ou, Y. et al. (2002) Neurogastroenterol Motil 14, 477-86.
  10. Meisler, M.H. and Kearney, J.A. (2005) J Clin Invest 115, 2010-7.
  11. Wallace, R.H. et al. (2001) Am J Hum Genet 68, 859-65.
  12. Auerbach, D.S. et al. (2013) PLoS One 8, e77843.
  13. Chopra, R. and Isom, L.L. (2014) Epilepsy Curr 14, 86-9.

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