Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Acetyl-α-Tubulin (Lys40) (D20G3) XP® Rabbit mAb (PE Conjugate) #18276

No. Size Price
18276S 100 µl ( 50 tests ) ¥4,264.00 现货查询 购买询价
18276 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
F 1:50 Human,Mouse,Rat,Monkey,Zebrafish, Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry,

Homology

Species predicted to react based on 100% sequence homology: Xenopus,

Specificity / Sensitivity

Acetyl-α-Tubulin (Lys40) (D20G3) XP® Rabbit mAb (PE Conjugate) detects endogenous levels of α-tubulin only when acetylated at Lys40. This amino acid is not conserved in β-tubulin.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic acetylpeptide corresponding to residues surrounding Lys40 of human α-tubulin.

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated Acetyl-α-Tubulin (Lys40) (D20G3) XP® Rabbit mAb #5335.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells untreated (blue) or treated with Trichostatin A (TSA) #9950 (green), using Acetyl-α-Tubulin (Lys40) (D20G3) XP® Rabbit mAb (PE Conjugate).

Background

The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).

  1. Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47.

Application References

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Protocols

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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