Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

CAR (D3W3G) Rabbit mAb #16984


No. Size Price
16984S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
16984 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 45-55 Rabbit IgG
IP 1:100
IHC-P 1:500
IF-IC 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

CAR (D3W3G) Rabbit mAb recognizes endogenous levels of total CAR protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg328 of human CAR protein.



Confocal immunofluorescent analysis of Hep G2 (left) and RD (right) cells, using CAR (D3W3G) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using CAR (D3W3G) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded Hep G2 (left) and K-562 (right) cell pellets using CAR (D3W3G) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using CAR (D3W3G) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).



Immunoprecipitation of CAR protein from HCT-15 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is CAR (D3W3G) Rabbit mAb. Western blot analysis was performed using CAR (D3W3G) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from HCT-15, LoVo, and HCC1419 cell lines using CAR (D3W3G) Rabbit mAb.


The coxsackie virus and adenovirus receptor (CXADR, CAR) is a highly conserved, single-transmembrane glycoprotein and the primary receptor to mediate cellular attachment and infection of coxsackie B viruses and most adenoviruses (1,2). The CAR protein contains a pair of Ig-like domains within the amino-terminal extracellular domain and a carboxyl-terminal PDZ motif (1). Research studies indicate that CAR is a tight junction protein that associates with the ZO-1 scaffold protein and promotes both cell adhesion and restriction of solute and ion movement between cells (2). Endogenous CAR is targeted to the basolateral plasma membrane by a tyrosine-based basolateral sorting signal and clathrin adaptors AP-1A and AP-1B (3). CAR binds junctional adhesion molecule L (JAML) on epithelial cells and neutrophils where it activates PI3K and downstream MAPK kinases to stimulate epithelial γδ T cell proliferation and increase production of TNFα and keratinocyte growth factor (4-6). As a result, the CAR protein plays a potentially critical role in adenoviral gene therapy, immunity, wound repair, inflammation, and cancer therapy (4-6). Additional studies demonstrate that CAR is essential in regulating squamous carcinoma cell growth (7).

  1. Bergelson, J.M. et al. (1997) Science 275, 1320-3.
  2. Cohen, C.J. et al. (2001) Proc Natl Acad Sci U S A 98, 15191-6.
  3. Carvajal-Gonzalez, J.M. et al. (2012) Proc Natl Acad Sci U S A 109, 3820-5.
  4. Zen, K. et al. (2005) Mol Biol Cell 16, 2694-703.
  5. Witherden, D.A. et al. (2010) Science 329, 1205-10.
  6. Verdino, P. et al. (2010) Science 329, 1210-4.
  7. Saito, K. et al. (2014) Oncogene 33, 1274-86.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


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For Research Use Only. Not For Use In Diagnostic Procedures.

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