Cell Signaling Technology

Product Pathways - Tag Antibodies

SV40 Large T Antigen (D1E9E) Rabbit mAb #15729

No. Size Price
15729S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
15729 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 All Species Expected, Recombinant 90 Rabbit IgG
IP 1:50
IF-IC 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

SV40 Large T Antigen (D1E9E) Rabbit mAb recognizes transgenic levels of total SV40 Large T Antigen protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys315 of SV40 Large T Antigen protein.

IF-IC

IF-IC

Confocal immunofluorescent analysis of 293T (positive, left) and HeLa (negative, right) cells, using SV40 Large T antigen (D1E9E) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red).

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 and 293T cells using SV40 Large T Antigen (D1E9E) Rabbit mAb (upper) and β-Tubulin (D2N5G) Rabbit mAb #15115 (lower).

Background

Simian Virus 40 (SV40) is a small double-stranded, circular DNA tumor virus encoding three tumor antigens: large T, Small T, and 17KT. These antigens contribute to cellular transformation, an area of research where they are utilized extensively. Transformation of cells by SV40 large T antigen is mediated by its binding to the tumor suppressor proteins RB and p53 in the nucleus. Additional binding partners include p300, CBP, Cul7, IRS1, Bub1, Nbs1, and Fbw7, which also contribute to transformation (1).

  1. Cheng, J. et al. (2009) Semin Cancer Biol 19, 218-28.

Application References

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Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

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Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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