Cell Signaling Technology

Product Pathways - Adhesion

MMP-9 (D6O3H) XP® Rabbit mAb (Biotinylated) #15561

mmp9  

No. Size Price
15561S 100 µl ( 10 western blots ) ¥4,264.00 现货查询 购买询价
15561 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 84,92 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

MMP-9 (D6O3H) XP® Rabbit mAb (Biotinylated) recognizes the full-length, proenzyme (92 kDa) and the cleaved, active enzyme (84 kDa) of MMP-9.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe542 of human MMP-9 protein.

Description

This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated MMP-9 (D6O3H) XP® Rabbit mAb #13667.

Western Blotting

Western Blotting

Western blot analysis of concentrated, serum-free cultured medium (5 μl) from U-2 OS cells, untreated (-) or treated with TPA (+) using MMP-9 (D6O3H) XP® Rabbit mAb (Biotinylated).

Background

The matrix metalloproteinases (MMPs) are a family of proteases that target many extracellular proteins including other proteases, growth factors, cell surface receptors, and adhesion molecules (1). Among the family members, MMP-2, MMP-3, MMP-7, and MMP-9 have been characterized as important factors for normal tissue remodeling during embryonic development, wound healing, tumor invasion, angiogenesis, carcinogenesis, and apoptosis (2-4). Research studies have shown that MMP activity correlates with cancer development (2). One mechanism of MMP regulation is transcriptional (5). Once synthesized, MMP exists as a latent proenzyme. Maximum MMP activity requires proteolytic cleavage to generate active MMPs by releasing the inhibitory propeptide domain from the full length protein (5).

  1. McCawley, L.J. and Matrisian, L.M. (2001) Curr Opin Cell Biol 13, 534-40.
  2. Coussens, L.M. et al. (2002) Science 295, 2387-92.
  3. Sternlicht, M.D. et al. (1999) Cell 98, 137-46.
  4. Vu, T.H. et al. (1998) Cell 93, 411-22.
  5. Nagase, H. et al. (1990) Biochemistry 29, 5783-9.

Application References

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Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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