Product Pathways - Chromatin Regulation / Epigenetics
HDAC4 (D8T3Q) Rabbit mAb #15164
|15164S||100 µl ( 10 western blots )||￥3,250.00 现货查询||购买询价|
|15164||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation,
Specificity / Sensitivity
HDAC4 (D8T3Q) Rabbit mAb recognizes endogenous levels of total HDAC4 protein. This antibody does not cross-react with other HDAC proteins, including HDAC5 and HDAC7.
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human HDAC4 protein.
Acetylation of the histone tail causes chromatin to adopt an "open" conformation, allowing increased accessibility of transcription factors to DNA. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription (1,2). HAT complexes interact with sequence-specific activator proteins to target specific genes. In addition to histones, HATs can acetylate nonhistone proteins, suggesting multiple roles for these enzymes (3). In contrast, histone deacetylation promotes a "closed" chromatin conformation and typically leads to repression of gene activity (4). Mammalian histone deacetylases can be divided into three classes on the basis of their similarity to various yeast deacetylases (5). Class I proteins (HDACs 1, 2, 3, and 8) are related to the yeast Rpd3-like proteins, those in class II (HDACs 4, 5, 6, 7, 9, and 10) are related to yeast Hda1-like proteins, and class III proteins are related to the yeast protein Sir2. Inhibitors of HDAC activity are now being explored as potential therapeutic cancer agents (6,7).
- Marmorstein, R. (2001) Cell Mol Life Sci 58, 693-703.
- Gregory, P.D. et al. (2001) Exp Cell Res 265, 195-202.
- Liu, Y. et al. (2000) Mol Cell Biol 20, 5540-53.
- Cress, W.D. and Seto, E. (2000) J Cell Physiol 184, 1-16.
- Gray, S.G. and Ekström, T.J. (2001) Exp Cell Res 262, 75-83.
- Thiagalingam, S. et al. (2003) Ann. N.Y. Acad. Sci. 983, 84-100.
- Vigushin, D.M. and Coombes, R.C. (2004) Curr Cancer Drug Targets 4, 205-18.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 12630 SignalFire™ Plus ECL Reagent
- 12757 SignalFire™ Elite ECL Reagent
- 3424 Phospho-HDAC4 (Ser632)/HDAC5 (Ser661)/HDAC7 (Ser486) Antibody
- 3443 Phospho-HDAC4 (Ser246)/HDAC5 (Ser259)/HDAC7 (Ser155) (D27B5) Rabbit mAb
- 3815 Phospho-HDAC3 (Ser424) Antibody
- 3949 HDAC3 (7G6C5) Mouse mAb
- 5113 HDAC2 (3F3) Mouse mAb
- 5356 HDAC1 (10E2) Mouse mAb
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7558 HDAC6 (D2E5) Rabbit mAb
- 7612 HDAC6 (D21B10) Rabbit mAb
- 7727 Biotinylated Protein Ladder Detection Pack
- 9998 BSA
- 9999 Nonfat Dry Milk
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Tween is a registered trademark of ICI Americas, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
用户评论 --- 共 0 条