Product Pathways - Protein Stability
VIMP (D1D1M) Rabbit mAb #15160
|15160S||100 µl ( 10 western blots )||￥3,250.00 现货查询||购买询价|
|15160||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation,
Specificity / Sensitivity
VIMP (D1D1M) Rabbit mAb recognizes endogenous levels of total VIMP protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys150 of human VIMP protein.
Western blot analysis of extracts from various cell lines using VIMP (D1D1M) Rabbit mAb.
Western blot analysis of extracts from 293T cells transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® VIMP siRNA I #13803 (+), or SignalSilence® VIMP siRNA II (+) #13818, using VIMP (D1D1M) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). The VIMP (D1D1M) Rabbit mAb confirms silencing of VIMP expression, while GAPDH (D16H11) XP® Rabbit mAb is used as a loading control.
VCP-interacting membrane protein (VIMP, selenoprotein S) is a putative reductase and endoplasmic reticulum (ER)-resident protein involved in the ER-associated degradation (ERAD) pathway (1,2). Research studies indicate that VIMP may play a protective role against inflammation and reduce ER-stress (3). The VIMP protein is a single-pass, transmembrane protein that recruits the cytosolic p97/VCP AAA-ATPase and its cofactors, UFD1 and NPL4, to the ER membrane (4). An ER membrane complex containing Derlin-1 and VIMP forms a critical node in the ERAD machinery and links substrate recognition in the ER lumen with the retrotranslocation function of the p97/VCP AAA-ATPase in the cytosol (1,4). Polymorphisms in the corresponding VIMP gene are associated with spontaneous preterm births and cardiovascular disease risk (5,6) while other studies do not support a correspondence between VIMP polymorphisms and inflammatory disorders (7).
- Lilley, B.N. and Ploegh, H.L. (2005) Proc Natl Acad Sci U S A 102, 14296-301.
- Christensen, L.C. et al. (2012) J Biol Chem 287, 26388-99.
- Fradejas, N. et al. (2011) Glia 59, 959-72.
- Ye, Y. et al. (2004) Nature 429, 841-7.
- Wang, Y. et al. (2013) PLoS One 8, e65657.
- Cox, A.J. et al. (2013) Acta Diabetol 50, 391-9.
- Martínez, A. et al. (2008) BMC Genomics 9, 329.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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For Research Use Only. Not For Use In Diagnostic Procedures.
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XP is a registered trademark of Cell Signaling Technology, Inc.
SignalSilence is a registered trademark of Cell Signaling Technology, Inc.
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Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
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