Cell Signaling Technology

Product Pathways - Protein Stability

VIMP (D1D1M) Rabbit mAb #15160

ERAD   SELENOS   SELS   SEPS1   UPR   VIMP  

No. Size Price
15160S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
15160 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 21 Rabbit IgG
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

VIMP (D1D1M) Rabbit mAb recognizes endogenous levels of total VIMP protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys150 of human VIMP protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using VIMP (D1D1M) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® VIMP siRNA I #13803 (+), or SignalSilence® VIMP siRNA II (+) #13818, using VIMP (D1D1M) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). The VIMP (D1D1M) Rabbit mAb confirms silencing of VIMP expression, while GAPDH (D16H11) XP® Rabbit mAb is used as a loading control.

IP

IP

Immunoprecipitation of VIMP from 293T cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is VIMP (D1D1M) Rabbit mAb. Western blot analysis was performed using VIMP (D1D1M) Rabbit mAb.

Background

VCP-interacting membrane protein (VIMP, selenoprotein S) is a putative reductase and endoplasmic reticulum (ER)-resident protein involved in the ER-associated degradation (ERAD) pathway (1,2). Research studies indicate that VIMP may play a protective role against inflammation and reduce ER-stress (3). The VIMP protein is a single-pass, transmembrane protein that recruits the cytosolic p97/VCP AAA-ATPase and its cofactors, UFD1 and NPL4, to the ER membrane (4). An ER membrane complex containing Derlin-1 and VIMP forms a critical node in the ERAD machinery and links substrate recognition in the ER lumen with the retrotranslocation function of the p97/VCP AAA-ATPase in the cytosol (1,4). Polymorphisms in the corresponding VIMP gene are associated with spontaneous preterm births and cardiovascular disease risk (5,6) while other studies do not support a correspondence between VIMP polymorphisms and inflammatory disorders (7).

  1. Lilley, B.N. and Ploegh, H.L. (2005) Proc Natl Acad Sci U S A 102, 14296-301.
  2. Christensen, L.C. et al. (2012) J Biol Chem 287, 26388-99.
  3. Fradejas, N. et al. (2011) Glia 59, 959-72.
  4. Ye, Y. et al. (2004) Nature 429, 841-7.
  5. Wang, Y. et al. (2013) PLoS One 8, e65657.
  6. Cox, A.J. et al. (2013) Acta Diabetol 50, 391-9.
  7. Martínez, A. et al. (2008) BMC Genomics 9, 329.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

SignalSilence is a registered trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications