Product Pathways - Lymphocyte Signaling
OX40 (D1S6L) Rabbit mAb (Pacific Blue™ Conjugate) #15147
|15147S||100 µl ( 50 tests )||￥3,876.00 现货查询||购买询价|
|15147||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: F=Flow Cytometry,
Specificity / Sensitivity
OX40 (D1S6L) Rabbit mAb (Pacific Blue™ Conjugate) recognizes endogenous levels of total OX40 protein. This antibody binds the intracellular domain of human OX40 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human OX40 protein.
This Cell Signaling Technology antibody is conjugated to Pacific Blue™ fluorescent dye and tested in-house for direct flow cytometry in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated antibody OX40 (D1S6L) Rabbit mAb #15123.
OX40 (TNFRSF4, CD134) is a member of the tumor necrosis factor (TNF) receptor superfamily that regulates T cell activity and immune responses. The OX40 protein contains four cysteine rich domains, a transmembrane domain, and a cytoplasmic tail containing a QEE motif (1,2). OX40 is primarily expressed on activated CD4+ and CD8+ T-cells, while the OX40 ligand (OX40L, TNFSF4, CD252) is predominantly expressed on activated antigen presenting cells (3-7). The engagement of OX40 with OX40L leads to the recruitment of TNF receptor-associated factors (TRAFs) and results in the formation of a TCR-independent signaling complex. One component of this complex, PKCθ, activates the NF-κB pathway (2,8). OX40 signaling through Akt can also enhance TCR signaling directly (9). Research studies indicate that the OX40L-OX40 pathway is associated with inflammation and autoimmune diseases (10). Additional research studies show that OX40 agonists augment anti-tumor immunity in several cancer types (11,12).
- Croft, M. (2010) Annu Rev Immunol 28, 57-78.
- So, T. and Croft, M. (2012) Front Immunol 3, 133.
- Paterson, D.J. et al. (1987) Mol Immunol 24, 1281-90.
- Mallett, S. et al. (1990) EMBO J 9, 1063-8.
- Dürkop, H. et al. (1995) Br J Haematol 91, 927-31.
- Godfrey, W.R. et al. (1994) J Exp Med 180, 757-62.
- Al-Shamkhani, A. et al. (1997) J Biol Chem 272, 5275-82.
- So, T. et al. (2011) Proc Natl Acad Sci U S A 108, 2903-8.
- So, T. and Croft, M. (2013) Front Immunol 4, 139.
- Gough, M.J. and Weinberg, A.D. (2009) Adv Exp Med Biol 647, 94-107.
- Weinberg, A.D. et al. (2011) Immunol Rev 244, 218-31.
- Linch, S.N. et al. (2015) Front Oncol 5, 34.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Pacific Blue is a trademark of Molecular Probes, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
用户评论 --- 共 0 条