Cell Signaling Technology

Product Pathways - Protein Stability

POMP (D2X9S) Rabbit mAb #15141

hUMP1   POMP   Proteassemblin  

No. Size Price
15141S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
15141 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 16 Rabbit IgG
IP 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Homology

Species predicted to react based on 100% sequence homology: Bovine,

Specificity / Sensitivity

POMP (D2X9S) Rabbit mAb recognizes endogenous levels of total POMP protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human POMP protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using POMP (D2X9S) Rabbit mAb.

IP

IP

Immunoprecipitation of POMP from RKO cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or POMP (D2X9S) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using POMP (D2X9S) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from RKO cells, untreated (-) or treated with Human Interferon-γ (hIFN-γ) #8901 (100 ng/ml, 72 hr; +), using POMP (D2X9S) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Interferon-γ induces increased POMP protein expression in RKO cells.

Background

The 26S proteasome is a highly abundant proteolytic complex involved in the degradation of ubiquitinated substrate proteins. It consists largely of the 20S catalytic core particle (CP) and the 19S/PA700 regulatory particle (RP) that caps either end of the CP. The CP consists of two stacked heteroheptameric β-rings (β1-7) that contain three catalytic β-subunits flanked on either side by two heteroheptameric α-rings (α1-7). The RP includes multimeric base and lid complexes. The RP base includes a heterohexameric ring of ATPase subunits that unfold the substrate and open the α-subunit gate to expose the substrate to the catalytic β-subunits. The lid consists of ubiquitin receptors and DUBs that recruit ubiquitinated substrates and modify ubiquitin chain topology (1,2). Proteasome activity modulators, such as PA28/11S REG, bind the 20S CP cylinder end and open the CP channel (1,2).

Proteasome maturation protein (POMP, proteassemblin, hUMP1) is an integral factor essential for assembly of the 20S catalytic core particle during mammalian proteasome biogenesis. POMP promotes heteroheptameric β-ring formation and dimerization of half-proteasomes during core particle assembly. The POMP protein undergoes proteasomal degradation following 20S CP complex assembly and activation (3-6). Research studies suggest that POMP is required for CP assembly for both constitutive proteasomes and immunoproteasomes, and that the assembly focal point resides at the endoplasmic reticulum (6-8). A single nucleotide deletion in the 5' UTR of POMP results in altered epidermal POMP distribution and the autosomal recessive skin disorder known as KLICK syndrome (9).

  1. Finley, D. (2009) Annu Rev Biochem 78, 477-513.
  2. Lee, M.J. et al. (2011) Mol Cell Proteomics 10, R110.003871.
  3. Griffin, T.A. et al. (2000) Mol Cell Biol Res Commun 3, 212-7.
  4. Witt, E. et al. (2000) J Mol Biol 301, 1-9.
  5. Ramos, P.C. et al. (1998) Cell 92, 489-99.
  6. Fricke, B. et al. (2007) EMBO Rep 8, 1170-5.
  7. Heink, S. et al. (2005) Proc Natl Acad Sci U S A 102, 9241-6.
  8. Heink, S. et al. (2006) Cancer Res 66, 649-52.
  9. Dahlqvist, J. et al. (2010) Am J Hum Genet 86, 596-603.

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For Research Use Only. Not For Use In Diagnostic Procedures.

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