Cell Signaling Technology

Product Pathways - Metabolism

TRXR1 (D1T3D) Rabbit mAb #15140

Gene associated with retinoic and IFN-induced mortality 12 protein   Gene associated with retinoic and interferon-induced mortality 12 protein   GRIM-12   GRIM12   KDRF   KM-102-derived reductase-like factor   MGC9145   oxidoreductase   Selenium   Selenocysteine   Selenoprotein   thioredoxin reductase 1   Thioredoxin reductase 1. cytoplasmic   thioredoxin reductase GRIM-12   Thioredoxin reductase TR1   TR1   TRXR1   TXNR   TXNRD1  

No. Size Price
15140S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
15140 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Hamster,Monkey, Endogenous 55 Rabbit IgG
IHC-P 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

TRXR1 (D1T3D) Rabbit mAb recognizes endogenous levels of total TRXR1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly412 of human TRXR1 protein.

Western Blotting

Western Blotting

Western blot analysis of Caco-2 cells, untreated (-) or treated (+) for 48 hr with R-sulforaphane (SFN; 10 μM) and sodium selenite (100 nM), using TRXR1 (D1T3D) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines and tissues using TRXR1 (D1T3D) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human testis using TRXR1 (D1T3D) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded Caco-2 cells, untreated (left) and R-sulforaphane (SFN) and sodium selenite-treated (right), using TRXR1 (D1T3D) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using TRXR1 (D1T3D) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using TRXR1 (D1T3D) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using TRXR1 (D1T3D) Rabbit mAb.

Background

TRXR1 (thioredoxin reductase 1) is a selenocysteine-containing protein that is involved in redox homeostasis (1-6). Its canonical target is thioredoxin, another redox protein (1). Together, they are involved in many functions such as antioxidant regulation (3-6), cell proliferation (2,3,5), DNA replication (2,3), and transcription (3,5). TRXR1 is also capable of reducing a wide array of cellular proteins (1,3). Selenium deficiency, either by diet modification (2,6) or introduction of methylmercury (4), hinders proper expression and function of TRXR1. It is possible that this effect, which results in a higher oxidative state, is a result of the selenocysteine codon (UGA) being read as a STOP codon in the absence of adequate selenium (4). The functions of TRXR1 in cell proliferation and antioxidant defense make it a potential therapeutic target.

  1. Turanov, A.A. et al. (2010) Biochem J 430, 285-93.
  2. Gasdaska, P.Y. et al. (1995) FEBS Lett 373, 5-9.
  3. Gromer, S. et al. (2004) Med Res Rev 24, 40-89.
  4. Usuki, F. et al. (2011) J Biol Chem 286, 6641-9.
  5. Pappas, A.C. et al. (2008) Comp Biochem Physiol B Biochem Mol Biol 151, 361-72.
  6. Müller, M. et al. (2010) Genes Nutr 5, 297-307.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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