Cell Signaling Technology

Product Pathways - Vesicle Trafficking

IGF-II Receptor/CI-M6PR (D8Z3J) Rabbit mAb #15128

No. Size Price
15128S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
15128 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 275 Rabbit IgG
IHC-P 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

IGF-II Receptor/CI-M6PR (D8Z3J) Rabbit mAb recognizes endogenous levels of total IGF-II receptor/CI-M6PR protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe1379 of human IGF-II receptor/CI-M6PR protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, SK-MEL-28, and NIH/3T3 cells using IGF-II Receptor/CI-M6PR (D8Z3J) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human placenta using IGF-II Receptor/CI-M6PR (D8Z3J) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded SK-OV-3 (left) and A549 (right) cell pellets using IGF-II Receptor/CI-M6PR (D8Z3J) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using IGF-II Receptor/CI-M6PR (D8Z3J) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human testes using IGF-II Receptor/CI-M6PR (D8Z3J) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human liver using IGF-II Receptor/CI-M6PR (D8Z3J) Rabbit mAb.

Background

Insulin-like growth factor II (IGF-II) receptor, also widely known as cation-independent mannose 6-phosphate receptor (CI-M6PR), is a multifunctional type I transmembrane glycoprotein that participates in the internalization of mannose-6-phosphate modified hydrolases and IGF-II from the plasma membrane (1,2). In the absence of ligands, IGF-II receptor is constitutively endocytosed from the cell surface to accumulate in the Golgi apparatus (3). In the presence of ligands, the receptor transports the mannose-6-phosphate modified hydrolases to acidified endosomes and lysosomes (4). The ligand-free receptor is then transported back to the Golgi compartment or the cell surface (4). In several research studies, IGF-II receptor has been recognized as a tumor suppressor in a number of cancers (5-7).

  1. Lobel, P. et al. (1989) Cell 57, 787-96.
  2. Kiess, W. et al. (1988) J Biol Chem 263, 9339-44.
  3. York, S.J. et al. (1999) J Biol Chem 274, 1164-71.
  4. Duncan, J.R. and Kornfeld, S. (1988) J Cell Biol 106, 617-28.
  5. Oates, A.J. et al. (1998) Breast Cancer Res Treat 47, 269-81.
  6. Martin-Kleiner, I. and Gall Troselj, K. (2010) Cancer Lett 289, 11-22.
  7. Puxbaum, V. et al. (2012) J Hepatol 57, 337-43.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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