Cell Signaling Technology

Product Pathways - Neuroscience

β-Amyloid (D3D2N) Mouse mAb #15126

sc-32277  

No. Size Price
15126S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
15126 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 5 Mouse IgG1
IHC-P 1:800
IF-F 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), IF-F=Immunofluorescence (Frozen),

Specificity / Sensitivity

β-Amyloid (D3D2N) Mouse mAb recognizes endogenous levels of total β-amyloid peptide (Aβ).

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human β-amyloid peptide (Aβ).

IF-F

IF-F

Confocal immunofluorescent analysis of brain sections from 13-month old wild-type (left) and Tg2576 (center) mice using β-Amyloid (D3D2N) Mouse mAb (green) and β3-Tubulin (D71G9) XP® Rabbit mAb #5568 (red). A detailed view of a cortical β-amyloid plaque is shown on the right. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of human Aβ-37, Aβ-40, and Aβ-42 peptides using β-Amyloid (D3D2N) Mouse mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human Alzheimer's brain using β-Amyloid (D3D2N) Mouse mAb.

Background

Amyloid β (Aβ) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms (1). The amino acid sequence of APP contains the amyloid domain, which can be released by a two-step proteolytic cleavage (1). The extracellular deposition and accumulation of the released Aβ fragments form the main components of amyloid plaques in Alzheimer's disease (1). APP can be phosphorylated at several sites, which may affect the proteolytic processing and secretion of this protein (2-5). Phosphorylation at Thr668 (a position corresponding to the APP695 isoform) by cyclin-dependent kinase is cell-cycle dependent and peaks during G2/M phase (4). APP phosphorylated at Thr668 exists in adult rat brain and correlates with cultured neuronal differentiation (5,6).

  1. Selkoe, D.J. (1996) J Biol Chem 271, 18295-8.
  2. Caporaso, G.L. et al. (1992) Proc Natl Acad Sci USA 89, 3055-9.
  3. Hung, A.Y. and Selkoe, D.J. (1994) EMBO J 13, 534-42.
  4. Suzuki, T. et al. (1994) EMBO J 13, 1114-22.
  5. Ando, K. et al. (1999) J Neurosci 19, 4421-7.
  6. Iijima, K. et al. (2000) J Neurochem 75, 1085-91.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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