Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

BRD7 (D9K2T) Rabbit mAb #15125

No. Size Price
15125S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
15125 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 85 Rabbit IgG
IHC-P 1:800

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

BRD7 (D9K2T) recognizes endogenous levels of total BRD7 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human BRD7 protein.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded MDA-MB-231 (left) and SW620 (right) cell pellets using BRD7 (D9K2T) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using BRD7 (D9K2T) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using BRD7 (D9K2T) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using BRD7 (D9K2T) Rabbit mAb.

Background

Bromodomain-containing protein 7 (BRD7, BP75, CELTIX-1) is a conserved bromodomain-containing protein that was first identified in a screen for proteins that interact with the PDZ domain of PSD95 (1). Subsequent studies identified BRD7 as a major component of SWI/SNF chromatin remodeling complexes, where it was shown to interact directly with acetylated histones to regulate gene transcription (2,3). BRD7 also interacts with p53, and was shown to participate directly in p53-dependent transcriptional regulation (4). Loss-of-function BRD7 mutations were identified in a subset of wild-type p53 breast cancer tumor samples, implicating BRD7 as a putative tumor-suppressor of potential clinical significance (5). BRD7 also associates with the BRCA1 protein, an interaction that facilitates recruitment of BRCA1 to the ERα gene promoter (6).

  1. Cuppen, E. et al. (1999) FEBS Lett 459, 291-8.
  2. Peng, C. et al. (2006) J Cell Biochem 97, 882-92.
  3. Kaeser, M.D. et al. (2008) J Biol Chem 283, 32254-63.
  4. Burrows, A.E. et al. (2010) Proc Natl Acad Sci U S A 107, 14280-5.
  5. Drost, J. et al. (2010) Nat Cell Biol 12, 380-9.
  6. Harte, M.T. et al. (2010) Cancer Res 70, 2538-47.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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