Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

β-Tubulin (D2N5G) Rabbit mAb #15115

sc-5274   sc-55529  

No. Size Price
15115S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
15115 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 55 Rabbit IgG
IHC-P 1:400
IF-IC 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

β-Tubulin (D2N5G) Rabbit mAb recognizes endogenous levels of total β-tubulin protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of human β-tubulin protein.

IF-IC

IF-IC

Confocal immunofluorescent analysis of NIH/3T3 cells using β-Tubulin (D2N5G) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using β-Tubulin (D2N5G) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3, and KNRK cells using β-Tubulin (D2N5G) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using β-Tubulin (D2N5G) Rabbit mAb.

Background

The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).

  1. Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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