Cell Signaling Technology

Product Pathways - Apoptosis

Smac/Diablo (D5S3R) Rabbit mAb #15108

No. Size Price
15108S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
15108 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 21 Rabbit IgG
IP 1:50
IHC-P 1:1000
F 1:100
IF-IC 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Smac/Diablo (D5S3R) Rabbit mAb recognizes endogenous levels of total Smac/Diablo protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Smac/Diablo protein.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with Z-VAD (50 μM, 3 hr) and Staurosporine #9953 (1 μM, 3 hr; right), using Smac/Diablo (D5S3R) Rabbit mAb. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Smac/Diablo (D5S3R) Rabbit mAb.

IP

IP

Immunoprecipitation of Smac/Diablo from Jurkat cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Smac/Diablo (D5S3R) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using Smac/Diablo Mouse mAb #2954.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells using Smac/Diablo (D5S3R) Rabbit mAb (blue) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded prostate carcinoma using Smac/Diablo (D5S3R) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded colon carcinoma using Smac/Diablo (D5S3R) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded lung carcinoma using Smac/Diablo (D5S3R) Rabbit mAb.

Background

Smac/Diablo is a 21 kDa mammalian mitochondrial protein that functions as a regulatory component during apoptosis (1,2). Upon mitochondrial stress, Smac/Diablo is released from mitochondria and competes with caspases for binding of IAPs (inhibitor of apoptosis proteins) (1,2). The interaction of Smac/Diablo with IAPs relieves the inhibitory effect of the IAPs on caspases (3,4). This interaction involves mainly the amino-terminal residues of Smac/Diablo with the BIR3 region of XIAP, supplemented with several other hydrophobic interactions between the helical structures of Smac/Diablo and other areas of BIR3 (5,6).

  1. Du, C. et al. (2000) Cell 102, 33-42.
  2. Verhagen, A.M. et al. (2000) Cell 102, 43-53.
  3. Srinivasula, S.M. et al. (2001) Nature 410, 112-6.
  4. Srinivasula, S.M. et al. (2000) J Biol Chem 275, 36152-7.
  5. Liu, Z. et al. (2000) Nature 408, 1004-8.
  6. Wu, G. et al. (2000) Nature 408, 1008-12.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

SignalStain is a trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications