Product Pathways - Apoptosis
Smac/Diablo (D5S3R) Rabbit mAb #15108
|15108S||100 µl ( 10 western blots )||￥3,250.00 现货查询||购买询价|
|15108||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),
Specificity / Sensitivity
Smac/Diablo (D5S3R) Rabbit mAb recognizes endogenous levels of total Smac/Diablo protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Smac/Diablo protein.
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with Z-VAD (50 μM, 3 hr) and Staurosporine #9953 (1 μM, 3 hr; right), using Smac/Diablo (D5S3R) Rabbit mAb. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Western blot analysis of extracts from various cell lines using Smac/Diablo (D5S3R) Rabbit mAb.
Immunoprecipitation of Smac/Diablo from Jurkat cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Smac/Diablo (D5S3R) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using Smac/Diablo Mouse mAb #2954.
Flow cytometric analysis of Jurkat cells using Smac/Diablo (D5S3R) Rabbit mAb (blue) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Immunohistochemical analysis of paraffin-embedded prostate carcinoma using Smac/Diablo (D5S3R) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded colon carcinoma using Smac/Diablo (D5S3R) Rabbit mAb.
Smac/Diablo is a 21 kDa mammalian mitochondrial protein that functions as a regulatory component during apoptosis (1,2). Upon mitochondrial stress, Smac/Diablo is released from mitochondria and competes with caspases for binding of IAPs (inhibitor of apoptosis proteins) (1,2). The interaction of Smac/Diablo with IAPs relieves the inhibitory effect of the IAPs on caspases (3,4). This interaction involves mainly the amino-terminal residues of Smac/Diablo with the BIR3 region of XIAP, supplemented with several other hydrophobic interactions between the helical structures of Smac/Diablo and other areas of BIR3 (5,6).
- Du, C. et al. (2000) Cell 102, 33-42.
- Verhagen, A.M. et al. (2000) Cell 102, 43-53.
- Srinivasula, S.M. et al. (2001) Nature 410, 112-6.
- Srinivasula, S.M. et al. (2000) J Biol Chem 275, 36152-7.
- Liu, Z. et al. (2000) Nature 408, 1004-8.
- Wu, G. et al. (2000) Nature 408, 1008-12.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
SignalStain is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
Tween is a registered trademark of ICI Americas, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
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