Cell Signaling Technology

Product Pathways - Transcription Factors

Aiolos (D1C1E) Rabbit mAb #15103

No. Size Price
15103S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
15103 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 50,58,65 Rabbit IgG
IP 1:200
IHC-P 1:50
F 1:100
ChIP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry, ChIP=Chromatin IP,

Specificity / Sensitivity

Aiolos (D1C1E) Rabbit mAb recognizes endogenous levels of total Aiolos protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro46 of human Aiolos protein.

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 Raji cells and either 5 μl of Aiolos (D1C1E) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human HSP90β Promoter Primers #12899, human SirT1 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

IP

IP

Immunoprecipitation of Aiolos from HT-29 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Aiolos (D1C1E) Rabbit mAb. Western blot analysis was performed using Aiolos (D1C1E) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Aiolos (D1C1E) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using Aiolos (D1C1E) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using Aiolos (D1C1E) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

Western Blotting

Western Blotting

Western blot analysis of extracts from U-937 cells and RPMI-8226 cells, untreated (-) or lenalidomide-treated (2 μM, 24 hr; +), using Aiolos (D1C1E) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8547 (lower).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of U-937 cells (blue), untreated RPMI-8226 cells (green), or lenalidomide-treated RPMI-8226 cells (2 μM, 24 hr; red), using Aiolos (D1C1E) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of fixed (2% formaldehyde) and permeabilized (0.1% Triton™ X-100) mouse splenocytes using Aiolos (D1C1E) Rabbit mAb and co-stained with a CD19 antibody. Anti-rabbit IgG (H+L), F(ab')2 Fragment (PE Conjugate) #8885 was used as a secondary antibody.

Background

Aiolos is an Ikaros family transcription factor composed of several zinc fingers that mediate DNA binding and homodimerization or heterodimerization with other Ikaros family members (1). Multiple Aiolos isoforms are generated through alternative splicing of the portion of the transcript encoding the amino-terminal zinc fingers (2). Aiolos is expressed by lymphoid tissues, with highest expression levels seen in mature B and T cells (1). The Ikaros family controls lymphocyte development by recruiting chromatin remodeling complexes to DNA (3). B cells from mice lacking Aiolos have a reduced threshold for activation, increased proliferation, elevated IgG and IgE, and develop B cell lymphomas (4). In T cells, Aiolos contributes to Th17 cell differentiation by suppressing IL-2 expression (5). Alterations in the Aiolos gene are observed in near haploid acute lymphoblastic leukemia, and the genetic locus containing Aiolos is linked to increased susceptibility to rheumatoid arthritis and systemic lupus erythematosus (6-8). Finally, aberrant expression of Aiolos in transformed epithelial cells promotes anchorage independence through downregulation of adhesion-related genes (9).

  1. Morgan, B. et al. (1997) EMBO J 16, 2004-13.
  2. Liippo, J. et al. (2001) Eur J Immunol 31, 3469-74.
  3. Kim, J. et al. (1999) Immunity 10, 345-55.
  4. Wang, J.H. et al. (1998) Immunity 9, 543-53.
  5. Quintana, F.J. et al. (2012) Nat Immunol 13, 770-7.
  6. Li, X. et al. (2014) Cancer Cell 25, 575-89.
  7. Holmfeldt, L. et al. (2013) Nat Genet 45, 242-52.
  8. Kurreeman, F.A. et al. (2012) Am J Hum Genet 90, 524-32.
  9. Lessard, C.J. et al. (2012) Am J Hum Genet 90, 648-60.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

SignalStain is a trademark of Cell Signaling Technology, Inc.

SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

Tween is a registered trademark of ICI Americas, Inc.

Triton is a trademark of The Dow Chemical Company.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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