Cell Signaling Technology

Product Pathways - Protein Stability

APC8 (D5O2D) Rabbit mAb #15100

ANAPC8   Anaphase Promoting Complex   APC-8   APC/C   APC/C TPR Subunits   APC8   CDC-23   CDC23   E3 Ubiquitin Ligase  

No. Size Price
15100S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
15100 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Monkey, Endogenous 64 Rabbit IgG
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

APC8 (D5O2D) Rabbit mAb recognizes endogenous levels of total APC8 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human APC8 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using APC8 (D5O2D) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from SCC-12 cells expressing either non-targeting shRNA (shNT) or APC8 shRNA (shAPC8), using APC8 (D5O2D) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human APC8 protein (hAPC8-Myc/DDK; +), using APC8 (D5O2D) Rabbit mAb.

IP

IP

Immunoprecipitation of APC8 from MOLT-4 cell extracts. Lane 1 is 10% input, lane 2 is APC8 (D5O2D) Rabbit mAb and lane 3 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900. Western blot analysis was performed using APC8 (D5O2D) Rabbit mAb.

Background

Eukaryotic cell proliferation depends strictly upon the E3 ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C), whose main function is to trigger the transition of the cell cycle from metaphase to anaphase. The APC/C complex promotes the assembly of polyubiquitin chains on substrate proteins in order to target these proteins for degradation by the 26S proteasome (1,2). The vertebrate APC/C complex consists of as many as 15 subunits, including multiple scaffold proteins, two catalytic subunits (APC2, APC11), and a number of proteins responsible for substrate recognition (3). All E3 enzymes, including APC/C, utilize ubiquitin residues activated by E1 enzymes and transferred to E2 enzymes. Research studies indicate that APC/C interacts with the E2 enzymes UBE2S and UBE2C via the RING-finger domain-containing subunit APC11 (4-6). APC/C function relies on multiple cofactors, including an APC/C coactivator formed by the cell division control protein 20 homolog (CDC20) and Cdh1/FZR1. The CDC20/Cdh1 coactivator is responsible for recognition of APC/C substrates through interaction with specific D-box and KEN-box recognition elements within these substrates (7-9).

Anaphase-promoting complex subunit 8 (APC8, CDC23) is a component of the tetratricopeptide repeat (TPR) APC/C sub-complex that also includes APC3 (CDC27) and APC6 (CDC16). APC8 protein associates with APC3 and APC6 to facilitate recruitment of the APC/C coactivation subunits CDC20 and Cdh1/FZR1 (10,11). Research studies suggest that APC8 protein is overexpressed in papillary thyroid cancer and acts as an important regulator of cell cycle progression and cell growth (12).

  1. Qiao, X. et al. (2010) Cell Cycle 9, 3904-12.
  2. Harper, J.W. et al. (2002) Genes Dev 16, 2179-206.
  3. Chang, L. et al. (2014) Nature 513, 388-93.
  4. Carroll, C.W. and Morgan, D.O. (2002) Nat Cell Biol 4, 880-7.
  5. Gmachl, M. et al. (2000) Proc Natl Acad Sci U S A 97, 8973-8.
  6. Leverson, J.D. et al. (2000) Mol Biol Cell 11, 2315-25.
  7. Kraft, C. et al. (2005) Mol Cell 18, 543-53.
  8. Glotzer, M. et al. (1991) Nature 349, 132-8.
  9. Pfleger, C.M. and Kirschner, M.W. (2000) Genes Dev 14, 655-65.
  10. Matyskiela, M.E. and Morgan, D.O. (2009) Mol Cell 34, 68-80.
  11. Thornton, B.R. et al. (2006) Genes Dev 20, 449-60.
  12. Zhang, L. et al. (2011) Endocr Relat Cancer 18, 731-42.

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For Research Use Only. Not For Use In Diagnostic Procedures.

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