Cell Signaling Technology

Product Pathways - Metabolism

DMT1/SLC11A2 (D3V8G) Rabbit mAb #15083

No. Size Price
15083S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
15083 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 55, 70-100 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

DMT1/SLC11A2 (D3V8G) Rabbit mAb recognizes endogenous levels of total DMT1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human DMT1 protein.

IP

IP

Immunoprecipitation of DMT1 from SK-MEL-5 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or DMT1/SLC11A2 (D3V8G) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using DMT1/SLC11A2 (D3V8G) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from SK-MEL-5 and SK-MEL-2 cells using DMT1/SLC11A2 (D3V8G) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from human SK-MEL-5 cells, untreated (-) or treated with PNGase F (+), using DMT1/SLC11A2 (D3V8G) Rabbit mAb (upper) and β-Actin (13E5) Rabbit mAb #4970 (lower).

Background

Divalent metal-ion transporter 1 (DMT1, SLC11A2, NRAMP2) is a transmembrane metal ion transport protein that plays critical roles in non-heme iron absorption in the intestine and iron acquisition by erythroid precursor cells (1,2). Following the cellular uptake of iron, DMT1 transfers ferric iron from the endosomes to the cytoplasm (3,4). The DMT1 protein can transport up to eight different metals, including iron, manganese, cobalt, and cadmium (5). Four mammalian DMT1 isoforms are expressed in various tissues and are differentially regulated at both the transcriptional and post-translational level (6,7). Mutations in the corresponding SLC11A2 gene can result in hypochromic microcytic anemia and iron overload. Aberrant iron transport in these individuals results in erythroid hyperplasia, high serum iron, and impaired liver function (8-10). Research studies show elevated DMT1 levels and iron accumulation in the substantia nigra of Parkinson's disease patients and the corresponding animal model (11,12).

  1. Gunshin, H. et al. (1997) Nature 388, 482-8.
  2. Canonne-Hergaux, F. et al. (2001) Blood 98, 3823-30.
  3. Canonne-Hergaux, F. et al. (1999) Blood 93, 4406-17.
  4. Hentze, M.W. et al. (2004) Cell 117, 285-97.
  5. Garrick, M.D. et al. (2003) Biometals 16, 41-54.
  6. Mackenzie, B. et al. (2007) Biochem J 403, 59-69.
  7. Garrick, M.D. et al. (2012) Biometals 25, 787-93.
  8. Mims, M.P. et al. (2005) Blood 105, 1337-42.
  9. Iolascon, A. et al. (2006) Blood 107, 349-54.
  10. Beaumont, C. et al. (2006) Blood 107, 4168-70.
  11. Salazar, J. et al. (2008) Proc Natl Acad Sci U S A 105, 18578-83.
  12. Howitt, J. et al. (2014) PLoS One 9, e87119.

Application References

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Protocols


For Research Use Only. Not For Use In Diagnostic Procedures.

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