Product Pathways - Tyrosine Kinase / Adaptors
PathScan® Total FGF Receptor 4 Sandwich ELISA Kit #15078
|Product Includes||Volume||Solution Color|
|ELISA Sample Diluent||25 ml||Blue|
|Anti-mouse IgG, HRP-linked Antibody (ELISA Formulated)||1 ea||Red (Lyophilized)|
|Detection Antibody Diluent||11 ml||Green|
|HRP Diluent||11 ml||Red|
|FGF Receptor 4 Mouse Detection mAb||1 ea||Green (Lyophilized)|
|STOP Solution||11 ml||Colorless|
|TMB Substrate #7004||11 ml||Colorless|
|ELISA Wash Buffer (20X)||25 ml||Colorless|
|Cell Lysis Buffer (10X) #9803||15 ml||Yellowish|
|FGFR4 Rabbit mAb Coated Microwells||1 ea|
|Sealing Tape||2 sheets|
Specificity / Sensitivity
PathScan® Total FGF Receptor 4 Sandwich ELISA Kit detects endogenous levels of FGFR4 protein in human cells, as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
The PathScan® Total FGF Receptor 4 Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of FGFR4 protein. An FGFR4 rabbit mAb has been coated onto the microwells. After incubation with cell lysates, both phospho- and nonphospho-FGFR4 proteins are captured by the coated antibody. Following extensive washing, an FGFR4 mouse detection mAb is added to detect captured FGFR4 proteins. Anti-mouse IgG, HRP-linked antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of absorbance for the developed color is proportional to the quantity of FGFR4 protein.
Antibodies in the kit are custom formulations specific to kit.
ELISA - Western correlation
Figure 1. FGF receptor 4 protein is detected from multiple cell lines using the PathScan® Total FGF Receptor 4 Sandwich ELISA Kit #15078. The absorbance readings at 450 nm are shown in the top figure, while the corresponding western blot using FGF Receptor 4 (D3B12) XP® Rabbit mAb #8562 is shown in the bottom figure.
Fibroblast growth factors (FGFs) produce mitogenic and angiogenic effects in target cells by signaling through cell surface receptor tyrosine kinases. There are four members of the FGF receptor family: FGFR1 (flg), FGFR2 (bek, KGFR), FGFR3, and FGFR4. Each receptor contains an extracellular ligand binding domain, a transmembrane domain, and a cytoplasmic kinase domain (1). Following ligand binding and dimerization, the receptors are phosphorylated at specific tyrosine residues (2). Seven tyrosine residues in the cytoplasmic tail of FGFR1 can be phosphorylated: Tyr463, 583, 585, 653, 654, 730, and 766. Tyr653 and Tyr654 are important for catalytic activity of activated FGFR and are essential for signaling (3). The other phosphorylated tyrosine residues may provide docking sites for downstream signaling components such as Crk and PLCγ (4,5).
- Powers, C.J. et al. (2000) Endocr Relat Cancer 7, 165-97.
- Reilly, J.F. et al. (2000) J Biol Chem 275, 7771-8.
- Mohammadi, M. et al. (1996) Mol Cell Biol 16, 977-89.
- Mohammadi, M. et al. (1991) Mol Cell Biol 11, 5068-78.
- Larsson, H. et al. (1999) J Biol Chem 274, 25726-34.
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- 2894 FGF Receptor 4 Antibody
- 69193 PathScan® Phospho-FGF Receptor 4 (panTyr) Sandwich ELISA Kit
- 7004 TMB Substrate
- 7076 Anti-mouse IgG, HRP-linked Antibody
- 8562 FGF Receptor 4 (D3B12) XP® Rabbit mAb
- 9803 Cell Lysis Buffer (10X)
- 9808 Phosphate Buffered Saline (PBS-20X)
- 9809 Phosphate Buffered Saline with Tween® 20 (PBST-20X)
- 9998 BSA
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
PathScan is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
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